详情页 - 聚合机构库演示平台

当前位置：首页 > 详情页

Genotoxicity and Reduced Heat Shock Protein 70 in Human Airway Smooth Muscle Cells Exposed to Cigarette Smoke Extract

117| 认领 | 导出 | 链接全文 |

文献详情

资源类型：

WOS体系：

Pubmed体系：

收录情况： ◇ SCIE

作者：

单位： [1]Department of Respiratory Medicine and Critical Care Medicine,Institute of Infectious Disease,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology [2]Department of Infectious Disease,Institute of Infectious Disease,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology

出处：

DOI：

关键词： airway smooth muscle cigarette smoke extract 8-hydroxydeoxyguanosine DNA damage heat shock protein 70

摘要：

Cigarette smoke is associated with the development of several diseases, such as chronic obstructive pulmonary disease (COPD). The purpose of this study was to investigate genotoxicity and heat shock protein 70 (Hsp70) in human airway smooth muscle cells (HASMCs) exposed to cigarette smoke extract (CSE). HASMCs was exposed to CSE with different doses for 24 h. The level of 8-hydroxydeoxyguanosine (8-OHdG) was determined by using HPLC-ECD, the DNA damage was analyzed by using comet assay, and apoptosis was examined by using Annexin-FITC/PI staining. The production of Hsp70 after CSE stimulation was tested. Results indicated that CSE significantly increased the level of 8-OHdG, DNA damage and cell apoptosis, and reduced the production of Hsp70. In particular, levels of Hsp70 were inversely correlated with 8-OHdG, DNA damage and cell apoptosis. It was concluded that cigarette smoke induced genotoxicity and decreased the production of cell protective protein Hsp70, which may contribute to the development of some airway diseases.

基金：

语种：

WOS：

PubmedID：

第一作者：

第一作者单位： [1]Department of Respiratory Medicine and Critical Care Medicine,Institute of Infectious Disease,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology

通讯作者：

推荐引用方式(GB/T 7714)：

APA：

MLA：

相关文献

[1]Genotoxicity and Reduced Heat Shock Protein 70 in Human Airway Smooth Muscle Cells Exposed to Cigarette Smoke Extract [2]Reduced heat shock protein 70 in airway smooth muscle in patients with chronic obstructive pulmonary disease [3]Cigarette smoke extract promotes proliferation of airway smooth muscle cells in asthmatic rats via regulating cyclin D1 expression. [4]Cigarette smoke extract promotes proliferation of airway smooth muscle cells in asthmatic rats via regulating cyclin D1 expression [5]周期蛋白D1在香烟提取物促进支气管哮喘大鼠气道平滑肌细胞增殖中的作用 [6]Heat shock cognate protein 70 gene is required for prevention of apoptosis induced by WSSV infection [7]Complexes of trophoblastic peptides and heat shock protein 70 as a novel contraceptive vaccine in a mouse model [8]Serum Heat Shock Protein 70 Concentration in Relation to Polycystic Ovary Syndrome in a Non-Obese Chinese Population [9]香烟烟雾提取物对支气管哮喘患者血清致敏的人气道平滑肌细胞增殖的影响 [10]2-phenylethynesulfonamide inhibits growth of oral squamous cell carcinoma cells by blocking the function of heat shock protein 70