To study the role of beta-actin in the signaling of transmembrane TNF-alpha (tmTNF-alpha)-mediated cytotoxicity, we mainly used bioassay and apoptosis assay, detection of the visual changes in beta-actin and intracellular translocation of signal molecules in response to tmTNF-alpha, and analysis of the signal molecules coupled or uncoupled with TNFR2 complex. These protocols might also be used to investigate the signaling events mediated by other transmembrane cytokines or members of TNF superfamily, which have transmembrane and soluble forms, as well as the involvement of actin cytoskeleton in signal transduction pathways.
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第一作者：
第一作者单位：[1]Wuhan Univ Sci & Technol, Coll Med, Dept Immunol, Wuhan, Hubei, Peoples R China
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推荐引用方式(GB/T 7714)：
Chen Hui,Leng Yan,Li Zhuoya.β-Actin in the Signaling of Transmembrane TNF-α-Mediated Cytotoxicity[J].TNF SUPERFAMILY: METHODS AND PROTOCOLS.2014,1155:55-68.doi:10.1007/978-1-4939-0669-7_6.
APA：
Chen, Hui,Leng, Yan&Li, Zhuoya.(2014).β-Actin in the Signaling of Transmembrane TNF-α-Mediated Cytotoxicity.TNF SUPERFAMILY: METHODS AND PROTOCOLS,1155,
MLA：
Chen, Hui,et al."β-Actin in the Signaling of Transmembrane TNF-α-Mediated Cytotoxicity".TNF SUPERFAMILY: METHODS AND PROTOCOLS 1155.(2014):55-68
