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Correlation of cytotoxic effect of transmembrane and secretory TNF-α to cell cycle

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单位： [1]Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Thyroid & Breast Surg, Wuhan 430030, Peoples R China [2]Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Basic Med Sci, Dept Immunol, Wuhan 430022, Peoples R China

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关键词： transmembrane TNF-alpha secretory TNF-alpha cytotoxicity cell apoptosis cell cycle

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This study was aimed to examine the correlation of the cytotoxic effects induced by two types of TNF-alpha to cell cycle. Hoechst 33342 and PI were used to detect the morphological changes in the cell death induced by the two types of TNF-alpha. TdT and PI co-staining was performed to determine the phase of cell cycle of apoptotic cells. L929 cells in different phases of cell cycle were further synchronized and their sensitivity to the two types of TNF-alpha was observed. Our results showed that the apoptosis of HepG2 cells triggered by tm-TNF-alpha mainly occurred in G(1) phase while in HL-60, Raji and K562 cell lines it mainly took place in S phase. The apoptosis of L929 cells induced by tm-TNF-alpha mainly occurred in S phase while the apoptosis induced by s-TNF-alpha mainly appeared in G(1) phase. L929 cells were sensitive to s-TNF-alpha when synchronized in G(1) phase (cytotoxicity 49.8%) while their sensitivity to tm-TNF-alpha was highest in S phase (45.7%) and G(1)/S phase (cytotoxicity 40.6%). It was concluded that tm-TNF-alpha-induced apoptosis of different target cells took place in different phases of cell cycle. The apoptosis of the specific cell line induced by the two types of TNF-alpha occurred in different phases of cell cycle. The sensitivity of the specific cell line to the two types of TNF-alpha was correlated with the phase of cell cycle.

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出版当年[2011]版：

大类 | 4 区医学

小类 | 4 区生化与分子生物学

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出版当年[2010]版：

Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

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影响因子： 0 最新[2023版] 0 最新五年平均 0.405 出版当年[2010版] 0 出版当年五年平均 0.311 出版前一年[2009版] 0.385 出版后一年[2011版]

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第一作者单位： [1]Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Thyroid & Breast Surg, Wuhan 430030, Peoples R China

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