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Immunofluorescent Study of Human MII Oocytes Failed to Fertilize after IVF and ICSI

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收录情况： ◇ 中华系列

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单位： [a]the center for reproductive medicine,tianjin central hospital of gynecology and obstetrics,tianjin,300052,china [b]department of gynecology and obstetrics,tongji hospital,tongji medical college,wuhan,430030,china

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关键词： fertilization failure immunofluorescence in vitro fertilization MII oocyte

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Objective: To discuss the reason why human MII oocytes failed to fertilize after IVF and ICSI. Methods: The unfertilized human MII oocytes were collected 24-48 h after IVF and ICSI and stained for immunoflurescence and PI counterstain. The types of fertilization failure were identified under the fluorescence microscopy. Results: About 55.8% oocytes in IVF were found no sperm in them, which were more than that in ICSI (9.7%) (P<0.01). About 14.9% oocytes in IVF and 58.1% in ICSI displayed oocyte activation failure. The difference was significant (P<0.01). Defects in pronuclear formation and/or migration was found in a similar proportion of oocytes both after IVF (25.3%) and ICSI (32.3%)(P>0.05). There were 3.9% oocytes with other abnormalities were observed in IVF but none in ICSI. Conclusion: The main reason of fertilization failure after IVF was no sperm penetration. However fertilization failure after ICSI was mainly associated with incomplete oocyte activation. © 2010 The Editorial Board of Journal of Reproduction and Contraception.

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第一作者：

第一作者单位： [a]the center for reproductive medicine,tianjin central hospital of gynecology and obstetrics,tianjin,300052,china [b]department of gynecology and obstetrics,tongji hospital,tongji medical college,wuhan,430030,china

通讯作者：

通讯机构： [a]the center for reproductive medicine,tianjin central hospital of gynecology and obstetrics,tianjin,300052,china [b]department of gynecology and obstetrics,tongji hospital,tongji medical college,wuhan,430030,china

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