Simultaneously reduced NKCC1 and Na,K-ATPase expression in murine cochlear lateral wall contribute to conservation of endocochlear potential following a sensorineural hearing loss
The mechanisms of the response in the murine cochlear lateral wall following sensorineural hearing loss (SNHL) are poorly understood. We focused on comparing the endocochlear potential (EP) with morphological changes in the lateral wall and expression of four important potassium (K+) transporters in a mouse model of SNHL induced by co-administration of aminoglycoside and loop diuretic. The expression of the alpha 1 and alpha 2 isoforms of Na,K-ATPase, Na-K-2C1-Cotransporter-1 (NKCC1) and potassium channel KCNQ1 was assessed. The EP showed a significant decline at 12 h post-treatment followed by complete recovery by 2 days post-treatment. The EP was maintained at near normal levels in animals deafened for periods up to 112 days. Despite this recovery, there was a significant and progressive decrease in the thickness of the stria vascularis, which was predominantly due to atrophy of marginal cells. Both protein and mRNA expression of alpha 1 and alpha 2 isoforms of Na,K-ATPase and NKCC1 in the lateral wall were dramatically reduced following a long-term deafening. KCNQ1 expression remained unchanged. These observations provide insight into the detailed mechanisms of EP modulation following SNHL and may have crucial implications in the future treatment of aminoglycoside-induced hearing loss. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
基金：
National Natural Science Foundation of China [30672307]
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Otolaryngol Head & Neck Surg, Wuhan 430030, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Xiong Hao,Chu Hanqi,Zhou Xiaoqin,et al.Simultaneously reduced NKCC1 and Na,K-ATPase expression in murine cochlear lateral wall contribute to conservation of endocochlear potential following a sensorineural hearing loss[J].NEUROSCIENCE LETTERS.2011,488(2):204-209.doi:10.1016/j.neulet.2010.11.030.
APA：
Xiong Hao,Chu Hanqi,Zhou Xiaoqin,Huang Xiaowen,Cui Yonghua...&Li Zhiyong.(2011).Simultaneously reduced NKCC1 and Na,K-ATPase expression in murine cochlear lateral wall contribute to conservation of endocochlear potential following a sensorineural hearing loss.NEUROSCIENCE LETTERS,488,(2)
MLA：
Xiong Hao,et al."Simultaneously reduced NKCC1 and Na,K-ATPase expression in murine cochlear lateral wall contribute to conservation of endocochlear potential following a sensorineural hearing loss".NEUROSCIENCE LETTERS 488..2(2011):204-209
