LaminB1, a major component of the nuclear lamina, is a potent regulator of cellular proliferation and senescence and also known to be essential for neuronal migration and brain development. However, the expression patterns of LaminB1 in the rat cochleae are still not fully revealed. Utilizing immunofluorescence, Western blotting, and quantitative real-time PCR, we identified the distribution and expression of LaminB1 in the rat cochleae. Immunofluorescence staining indicated that LaminB1 was mainly localized in the auditory hair cells (HCs), spiral ganglion cells (SGC), stria vascularis (STV, including spiral ligament), Reissner's membrane (RM), and limbus laminae spiralis (LLS). Western blotting analysis illustrated that the distribution of LaminB1 in rat cochleae was characterized by tissue specificity. The LaminB1 protein was expressed more in SGC and basilar membrane (BM) than in STV. Meanwhile, the mRNA expression of LaminB1 displayed difference in cochlear tissues. These observations preliminarily revealed the expression patterns of LaminB1, providing a theoretical basis for further study on the role of LaminB1 in auditory function.
基金：
National Natural Science Foundation of China [81771004, 81500791, 81300827]
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Otolaryngol Head & Neck Surg, Wuhan 430030, Hubei, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Du Zhi-hui,Chen Jin,Chen Qing-guo,et al.Expression Patterns and Implications of LaminB1 in Rat Cochleae[J].CURRENT MEDICAL SCIENCE.2019,39(2):305-309.doi:10.1007/s11596-019-2035-1.
APA：
Du, Zhi-hui,Chen, Jin,Chen, Qing-guo,Zhou, Liang-qiang,Bing, Dan...&Chu, Han-qi.(2019).Expression Patterns and Implications of LaminB1 in Rat Cochleae.CURRENT MEDICAL SCIENCE,39,(2)
MLA：
Du, Zhi-hui,et al."Expression Patterns and Implications of LaminB1 in Rat Cochleae".CURRENT MEDICAL SCIENCE 39..2(2019):305-309
