The purpose of this study was to investigate the mechanism of glioma cell invasion in hypoxic conditions. We demonstrated that hypoxia increased cell invasion, matrix metalloproteinase-2 (MMP2) activity and time-dependent expression of hypoxia inducible factor-1 alpha (HIF-1 alpha) in human glioma cells. These data suggest that MMP2 may play a significant role in tumor invasion in hypoxic conditions. We investigated the mechanisms involved in the increased MMP2 activity and cell invasion in hypoxic conditions. Increased expression of phospho-Jun NH2-terminal kinase (p-JNK) and phospho-c-Jun (p-c-Jun) in glioma cells induced by hypoxia was detected. Furthermore, this effect may be reduced by inhibiting the JNK signaling pathway. We found that inhibition of RhoA geranylgeranylation by geranylgeranyltransferase inhibitor-2147 (GGTI-2147) or knockdown of RhoA by si RNA against RhoA reduced the expression of p-JNK and p-c-Jun, and decreased MMP2 activity and glioma cell invasion in hypoxic conditions. These data suggest a link among RhoA, JNK, c-Jun and MMP2 activity that is functionally involved in the increased glioma cell invasion induced by hypoxia.
第一作者单位：[1]Huazhong Univ Sci & Technol,Dept Neurosurg,Tongji Hosp,Tongji Med Coll,Wuhan 430030,Hubei,Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Tong Jiao Jian,Yan Zhang,Jian Ren,et al.RhoA regulates invasion of glioma cells via the c-Jun NH2-terminal kinase pathway under hypoxia[J].ONCOLOGY LETTERS.2012,4(3):495-500.doi:10.3892/ol.2012.777.
APA：
Tong, Jiao Jian,Yan, Zhang,Jian, Ren,Tao, Huang,Hui, Ouyang Tao&Jian, Chen.(2012).RhoA regulates invasion of glioma cells via the c-Jun NH2-terminal kinase pathway under hypoxia.ONCOLOGY LETTERS,4,(3)
MLA：
Tong, Jiao Jian,et al."RhoA regulates invasion of glioma cells via the c-Jun NH2-terminal kinase pathway under hypoxia".ONCOLOGY LETTERS 4..3(2012):495-500
