Extracellular hypotonic stress can affect cellular function. Whether and how hypotonicity affects immune cell function remains to be elucidated. Macrophages are immune cells that play key roles in adaptive and innate in immune reactions. The purpose of this study was to investigate the role and underlying mechanism of hypotonic stress in the function of bone marrow-derived macrophages (BMDMs). Hypotonic stress increased endocytic activity in BMDMs, but there was no significant change in the expression of CD80, CD86, and MHC class II molecules, nor in the secretion of TNF-alpha or IL-10 by BMDMs. Furthermore, the enhanced endocytic activity of BMDMs triggered by hypotonic stress was significantly inhibited by chloride channel-3 (ClC-3) siRNA. Our findings suggest that hypotonic stress can induce endocytosis in BMDMs and that ClC-3 plays a central role in the endocytic process.
基金：
National Natural Science Foundation of China [81273221]; Major State Basic Research Development Program of China (973 Program) [2013CB530505]
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Immunol, Wuhan 4340030, Peoples R China[2]Huazhong Univ Sci & Technol,Tongji Med Coll,Tongji Hosp,Inst Organ Transplantat,Wuhan 4340030,Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Yan Yutao,Ding Yu,Ming Bingxia,et al.Increase in Hypotonic Stress-Induced Endocytic Activity in Macrophages via ClC-3[J].MOLECULES AND CELLS.2014,37(5):418-425.doi:10.14348/molcells.2014.0031.
APA：
Yan, Yutao,Ding, Yu,Ming, Bingxia,Du, Wenjiao,Kong, Xiaoling...&Gong, Feili.(2014).Increase in Hypotonic Stress-Induced Endocytic Activity in Macrophages via ClC-3.MOLECULES AND CELLS,37,(5)
MLA：
Yan, Yutao,et al."Increase in Hypotonic Stress-Induced Endocytic Activity in Macrophages via ClC-3".MOLECULES AND CELLS 37..5(2014):418-425
