To gain insights into the effect of MexB gene under the short interfering RNA (siRNA), we synthesized 21 bp siRNA duplexes against the MexB gene. RT-PCR was performed to determine whether the siRNA inhibited the expression of MexB mRNA. Changes in antibiotic susceptibility in response to siRNA were measured by the E-test method. The efficacy of siRNAs was determined in a murine model of chronic P. aeruginosa lung infection. MexB-siRNAs inhibited both mRNA expression and the activity of P. aeruginosa in vitro. In vivo, siRNA was effective in reducing the bacterial load in the model of chronic lung infection and the P. aeruginosa-induced pathological changes. MexB-siRNA treatment enhanced the production of inflammatory cytokines in the early infection stage (P < 0.05). Our results suggest that targeting of MexB with siRNA appears to be a novel strategy for treating P. aeruginosa infections.
基金：
National Natural Science Foundation of China [30873189]
第一作者单位：[1]Huazhong Univ Sci & Technol, Wuhan Pu Ai Hosp, Tongji Med Coll, Dept Infect Dis, Wuhan 430030, Peoples R China[3]Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Infect Dis, Wuhan 430030, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Gong Feng-Yun,Zhang Ding-Yu,Zhang Jiang-Guo,et al.siRNA-mediated gene silencing of MexB from the MexA-MexB-OprM efflux pump in Pseudomonas aeruginosa[J].BMB REPORTS.2014,47(4):203-208.doi:10.5483/BMBRep.2014.47.4.040.
APA：
Gong, Feng-Yun,Zhang, Ding-Yu,Zhang, Jiang-Guo,Wang, Li-Li,Zhan, Wei-Li...&Song, Jian-Xin.(2014).siRNA-mediated gene silencing of MexB from the MexA-MexB-OprM efflux pump in Pseudomonas aeruginosa.BMB REPORTS,47,(4)
MLA：
Gong, Feng-Yun,et al."siRNA-mediated gene silencing of MexB from the MexA-MexB-OprM efflux pump in Pseudomonas aeruginosa".BMB REPORTS 47..4(2014):203-208
