Background: Anatomical reconstruction using a semitendinosus tendon autograft is one of the most widely-used techniques for chronic lateral ankle instability (CLAI), and it can result in good biomechanical recovery for patients. The purpose of this study was to investigate the outcome of a novel individualized three-dimensional printed guide template for lateral ankle ligament reconstruction compared with the traditional surgical methods. Material/Methods: We retrospectively studied 34 patients with CLAI who required lateral ankle ligament reconstruction. Patients were randomly divided into 2 cohorts: the template group (18 patients) and the conventional group (16 patients). The average operation duration and number of radiation exposures were compared between the 2 cohorts. The displacement of anterior talar and talar tilt angle were recorded at the last follow-up, and Karlsson-Peterson score and American Orthopedic Foot and Ankle Society Score (AOFAS) were also compared. Results: All patients had satisfactory ankle stability at the last follow-up. The average operation duration was 51.9 +/- 3.6 min and the average number of radiation exposures was 1.34 +/- 0.6 in the template group, and the average operation duration was 72.4 +/- 12.6 min and the average number of radiation exposures was 6.58 +/- 1.7 in the conventional group. Difference between the 2 cohorts was statistically significant. However, in AOFAS (95.2 +/- 2.5 vs. 94.9 +/- 2.2; P>0.01.) and Karlsson Score (94.7 +/- 3.6 vs. 93.8 +/- 4.1; P>0.01.), no significant differences were found between the 2 cohorts. Conclusions: Both the template technique and the conventional method provided satisfactory outcomes for CLAI patients. However, the shorter operation duration and low number of radiation exposures in the template cohort suggest it is the better alternative for treatment of CLAI.