BackgroundThis study investigated the role of the ferroptosis-related gene FTH1 in oral squamous cell carcinoma (OSCC) and evaluated the therapeutic potential of baicalin in OSCC cell treatment.MethodsA prognostic model was established by bioinformatic analysis, consisting of 12 ferroptosis related genes (FRGs), and FTH1 was selected as the most significantly up-regulated FRGs. The clinical correlation of FTH1 in OSCC samples was evaluated by both immunohistochemical and bioinformatic characterizations. The effects of FTH1 on migration, invasion, epithelial-mesenchymal transition (EMT) and proliferation were determined by wound healing assays, transwell assays, western blotting and 5 '-ethynl 2 '-deoxyuridine proliferation assays, respectively. The effects of FTH1 on ferroptosis were tested via ferroptosis markers and Mito Tracker staining. In addition, the therapeutic effects of baicalin on OSCC cells were confirmed using EMT, migration, invasion, proliferation and ferroptosis assays.ResultsThe 12 FRGs were predictive of the prognosis for OSCC patients, and FTH1 expression was identified as significantly up-regulated in OSCC samples, which was highly associated with survival, immune cell infiltration and drug sensitivity. Moreover, knocking down FTH1 inhibited cell proliferation, EMT and invasive phenotypes, but induced ferroptosis in OSCC cells (Cal27 and SCC25). Furthermore, baicalin directly suppressed expression of FTH1 in OSCC cells, and effectively promoted ferroptosis and inhibited the proliferation as well as EMT by directly targeting FTH1.ConclusionsThis study has demonstrated that FTH1 is a therapeutic target for OSCC treatment, and has provided evidence that baicalin offers a promising alternative for OSCC treatment. In oral squamous cell carcinoma (OSCC), the ferroptosis-related gene FTH1 is overexpressed, promoting cell proliferation and epithelial-mesenchymal transition (EMT), while concurrently suppressing ferroptosis. Baicalin, by down-regulating FTH1, mitigates these effects, reducing EMT and proliferation and facilitating ferroptosis in OSCC cells. (Created by Biorender) image
基金：
This study was funded by the Hubei Provincial
Natural Science Foundation of China
(no. 2023AFB727, to X.C.)
语种：
外文
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2023]版：
大类|4 区医学
小类|3 区生物工程与应用微生物4 区遗传学4 区医学：研究与实验
最新[2025]版：
大类|4 区医学
小类|3 区生物工程与应用微生物4 区遗传学4 区医学：研究与实验
JCR分区：
出版当年[2022]版：
Q2BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ2GENETICS & HEREDITYQ3MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q2BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ2GENETICS & HEREDITYQ2MEDICINE, RESEARCH & EXPERIMENTAL
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Stomatol, Wuhan, Peoples R China
通讯作者：
通讯机构：[5]Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Oncol, Wuhan, Peoples R China[6]Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Oncol, Wuhan 430030, Peoples R China
推荐引用方式(GB/T 7714)：
Wen Zhihao,Zhang Yuxiao,Gao Bo,et al.Baicalin induces ferroptosis in oral squamous cell carcinoma by suppressing the activity of FTH1[J].JOURNAL OF GENE MEDICINE.2024,26(2):doi:10.1002/jgm.3669.
APA：
Wen, Zhihao,Zhang, Yuxiao,Gao, Bo&Chen, Xin.(2024).Baicalin induces ferroptosis in oral squamous cell carcinoma by suppressing the activity of FTH1.JOURNAL OF GENE MEDICINE,26,(2)
MLA：
Wen, Zhihao,et al."Baicalin induces ferroptosis in oral squamous cell carcinoma by suppressing the activity of FTH1".JOURNAL OF GENE MEDICINE 26..2(2024)
