Gamete and embryo quality are critical to the success rate of Assisted Reproductive Technology (ART) cycles, but there remains a lack of methods to accurately measure the quality of sperm, oocytes and embryos. The ability of Artificial Intelligence (AI) technology to analyze large amounts of data, especially video and images, is particularly useful in gamete and embryo assessment and selection. The well-trained model has fast calculation speed and high accuracy, which can help embryologists to perform more objective gamete and embryo selection. Various artificial intelligence models have been developed for gamete and embryo assessment, some of which exhibit good performance. In this review, we summarize the latest applications of AI technology in semen analysis, as well as selection for sperm, oocyte and embryo, and discuss the existing problems and development directions of artificial intelligence in this field.