单位：[1]Neurological Department,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China.华中科技大学同济医学院附属同济医院神经科[2]Hubei Key Laboratory of Neural Injury and Functional Reconstruction, Huazhong University of Science and Technology, Wuhan 430030, China.[3]Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430070, China.[4]MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan 430070, China.
Due to the heterogeneity of amyloid β-42 (Aβ42) species, the potential correlation between plasma oligomeric Aβ42 (oAβ42) and cognitive impairments in cerebral small vessel disease (CSVD) remains unclear. Herein, a sandwich ELISA for the specific detection of Aβ42 oligomers (oAβ42) and total Aβ42 (tAβ42) was developed based on sequence- and conformation-specific antibody pairs for the evaluation of plasma samples from a Chinese CSVD community cohort. After age and gender matching, 3-Tesla magnetic resonance imaging and multidimensional cognitive assessment were conducted in 134 CSVD patients and equal controls. The results showed that plasma tAβ42 and oAβ42 levels were significantly elevated in CSVD patients. By regression analysis, these elevations were correlated with the presence of CSVD and its imaging markers (i.e., white matter hyperintensities). Plasma Aβ42 tests further strengthened the predictive power of vascular risk factors for the presence of CSVD. Relative to tAβ42, oAβ42 showed a closer correlation with memory domains evaluated by neuropsychological tests. In conclusion, this sensitive ELISA protocol facilitated the detection of plasma Aβ42; Aβ42, especially its oligomeric form, can serve as a biosensor for the presence of CSVD and associated cognitive impairments represented by memory domains.
基金：
National Nature Science Foundation of China (82171385 to
Xiang Luo; 81971025 to Haiming Luo); Key Research and Development Program of Hubei Province
(2020BCA070 to Xiang Luo); the Application Foundation Frontier Special Project ofWuhan Science
and Technology Bureau (2020020601012226 to Xiang Luo); and the Flagship Program of Tongji
Hospital (2019CR106 to Xiang Luo).
第一作者单位：[1]Neurological Department,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China.[2]Hubei Key Laboratory of Neural Injury and Functional Reconstruction, Huazhong University of Science and Technology, Wuhan 430030, China.
共同第一作者：
通讯作者：
通讯机构：[1]Neurological Department,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China.[2]Hubei Key Laboratory of Neural Injury and Functional Reconstruction, Huazhong University of Science and Technology, Wuhan 430030, China.[3]Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430070, China.[4]MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan 430070, China.
推荐引用方式(GB/T 7714)：
Qu Wensheng,Zhang Liding,Liang Xiaohan,et al.Elevated Plasma Oligomeric Amyloid -42 Is Associated with Cognitive Impairments in Cerebral Small Vessel Disease[J].BIOSENSORS-BASEL.2023,13(1):doi:10.3390/bios13010110.
APA：
Qu Wensheng,Zhang Liding,Liang Xiaohan,Yu Zhiyuan,Huang Hao...&Luo Xiang.(2023).Elevated Plasma Oligomeric Amyloid -42 Is Associated with Cognitive Impairments in Cerebral Small Vessel Disease.BIOSENSORS-BASEL,13,(1)
MLA：
Qu Wensheng,et al."Elevated Plasma Oligomeric Amyloid -42 Is Associated with Cognitive Impairments in Cerebral Small Vessel Disease".BIOSENSORS-BASEL 13..1(2023)
