ObjectiveThis study describes a new approach to regenerate bone defect using autogenous tooth. Materials and methodsFreshly extrac-ted teeth were used as autogenous grafts. Teeth were sectioned, cut into desired shape, and disinfected. The grafts were rigidly fixed to the mandibular defects in eighteen rabbits using titanium screws to achieve good stability. Every six rabbits were stochastically sacrificed at 1, 3, and 6months after implantation, respectively. For all specimens, clinical, radiographical, and histological measurements were performed. ResultsThe boundaries of the grafts were distinctly visible in the implanted area during the first and third month. However, the teeth grafts were fully covered by new bone by the sixth month. The radiograph demonstrated the progressive change in the bone and grafted tooth interface from radiolucency to radiopacity during different time periods. Histologically, vascularization led to a temporary fibrous integration in the graft-bone interface. The bone contact rate of 1 and 3months was significantly lower than that of the 6months. During this period, grafts were gradually resorbed and replaced by new bone. ConclusionRigid fixation of autogenous tooth could serve as a novel approach for the repair of bone defect.