Objectives: This study aimed to evaluate the potential of the extrafibrillar dentin demineralization strategy on the long-term dentin bond strength of an etch-and-rinse adhesive.Methods: A water-soluble glycol chitosan-EDTA (GCE), a chelating conditioner, was synthesized and subjected to size-exclusion dialysis to obtain molecules >40 kDa. The conjugation of EDTA to glycol chitosan was analyzed by Fourier transform infrared (FTIR) spectroscopy. Mid-coronal dentin surfaces of 80 teeth were either acid-etched with 35% phosphoric acid or conditioned with 25 mg/mL GCE (n = 40) and thoroughly water-sprayed before applying the etch-and-rinse adhesive Adper Single Bond Plus and placing Z250 composite resin (3 M Oral Care; St Paul, MN, USA). Resin-bonded specimens were prepared into beams with a cross-sectional area of about 0.9 mm2 vertically through the resin-dentin interfaces before the microtensile bond strengths (MTBS) were determined immediately or after 3, 6, or 12 months of water storage. The resin-dentin interfaces were analyzed using transmission electron microscopy (TEM). The MTBS data were analyzed using two-way ANOVA followed by the LSD post-hoc multiple comparisons (P < 0.05).Results: FTIR spectra showed that EDTA was successfully conjugated to glycol chitosan. The phosphoric acidetching group and GCE-conditioning group showed similar bond strength values after 24 h of water storage. The bond strength of the phosphoric acid-etching group after 12-month water aging was significantly reduced from 51.61 +/- 3.30 MPa to 38.57 +/- 4.81 MPa, while the bond strength of the GCE-conditioning group was not significantly reduced from 50.28 +/- 3.62 MPa to 46.40 +/- 4.71 MPa.The degradation of the hybrid layer could be detected in the phosphoric acid-etching group after 12 months of water aging, but not in the GCE-conditioning group.Conclusion: The extrafibrillar dentin demineralization strategy using GCE conditioner could defy the hybrid layer degradation of the dentin bond after 12 months of water aging and enhance the dentin bond durability of the etch-and-rinse adhesive Adper Single Bond Plus.
基金：
National Natural Science Foundation of China [81970982]; Natural Scientific Research Fund of the Zhejiang Province, China [LGF20H140009]; Medical and Health Science and Technology Plan Project of the Zhejiang Province, China [2020PY057]
第一作者单位：[1]Zhejiang Univ, Zhejiang Univ Sch Med, Stomatol Hosp,Sch Stomatol, Clin Res Ctr Oral Dis Zhejiang Prov,Key Lab Oral, Hangzhou, Peoples R China
通讯作者：
通讯机构：[1]Zhejiang Univ, Zhejiang Univ Sch Med, Stomatol Hosp,Sch Stomatol, Clin Res Ctr Oral Dis Zhejiang Prov,Key Lab Oral, Hangzhou, Peoples R China[*1]Zhejiang Univ, Zhejiang Univ Sch Med, Stomatol Hosp,Sch Stomatol, Clin Res Ctr Oral Dis Zhejiang Prov,Key Lab Oral, Yanan Rd 395, Hangzhou 310006, Zhejiang, Peoples R China
推荐引用方式(GB/T 7714)：
Li Ming Xing,Duan Lian,Chen Mei Ling,et al.Effect of an extrafibrillar dentin demineralization strategy on the durability of the resin-dentin bond[J].JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS.2022,126:doi:10.1016/j.jmbbm.2021.105038.
APA：
Li, Ming Xing,Duan, Lian,Chen, Mei Ling,Tian, Fu Cong&Fu, Bai Ping.(2022).Effect of an extrafibrillar dentin demineralization strategy on the durability of the resin-dentin bond.JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS,126,
MLA：
Li, Ming Xing,et al."Effect of an extrafibrillar dentin demineralization strategy on the durability of the resin-dentin bond".JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS 126.(2022)
