单位：[1]Department of Integrated Traditional Chinese and Western Medicine,Tongji hospital,Huazhong University of Science and Technology,Jiefang Road 1095#,430030 Wuhan,China.华中科技大学同济医学院附属同济医院中医科[2]Institute of Integrated Traditional Chinese and Western Medicine,Tongji hospital,Huazhong University of Science and Technology,Jiefang Road 1095 #,430030 Wuhan,China.华中科技大学同济医学院附属同济医院中西医结合研究所[3]Department of Rehabilitation Center of Wuhan Puren Hospital, Affiliated Hospital of Wuhan, University of Science and Techn ology, Benxi Street 1#, Qingshan District, 430081 Wuhan, China.
Background To explore the development mechanism of PCOS and Transcriptomics was applied to seek the key gene. Methods Transcriptomics marked by UID (unique identifier) technique of granulosa cell in PCOS and control women was carried out and key gene was picked up. Then the key gene in granulosa cell was measured by RT-PCR. Two PCOS models modeling with Letrozole and Testosterone Propionate were implemented and the key gene in granulosa cell of ovary was measured by immunohistochemistry to verify the relation with PCOS. Results GO-enrich of transcriptomics concentrated in domain steroid metabolism and domain mitochondria. Different genes were sought from coexisting in both domain steroid metabolism and domain mitochondria. Finally, five different genes including CYP11A1?CYB5R1?STAR?FDX1 and AMACR were obtained. RT-PCR was implemented to furtherly verify the downregulating mRNA of FDX1 in PCOS, which showed the consistent outcome with the transcriptomics. Level of FDX1 protein in granulosa cell of antral follicle in two PCOS models was measured and decreased. Conclusions FDX1 was related with steroid metabolism and mitochondrial and may participate in the development of PCOS.
基金：
National Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [81573787, 81673757, 81874382]
第一作者单位：[1]Department of Integrated Traditional Chinese and Western Medicine,Tongji hospital,Huazhong University of Science and Technology,Jiefang Road 1095#,430030 Wuhan,China.
通讯作者：
推荐引用方式(GB/T 7714)：
wang zhi,dong hui,yang li,et al.The role of FDX1 in granulosa cell of Polycystic ovary syndrome (PCOS)[J].BMC ENDOCRINE DISORDERS.2021,21(1):doi:10.1186/s12902-021-00775-w.
APA：
wang,zhi,dong,hui,yang,li,yi,ping,wang,qing&huang,dongmei.(2021).The role of FDX1 in granulosa cell of Polycystic ovary syndrome (PCOS).BMC ENDOCRINE DISORDERS,21,(1)
MLA：
wang,zhi,et al."The role of FDX1 in granulosa cell of Polycystic ovary syndrome (PCOS)".BMC ENDOCRINE DISORDERS 21..1(2021)
