AIM: To investigate the antifibrotic effect of freeze-dried bilayered fibrin-binding amniotic membrane on trabeculectomy in a rabbit model. METHODS: Twenty-four Japanese white rabbits were randomized into three groups: the experimental group (ocular trabeculectomy in combination with freeze-dried bilayered fibrin-binding amniotic membrane transplantation), the control group (ocular trabeculectomy in combination with natural bilayered fibrin-binding amniotic membrane) and the blank group (single trabeculectomy). Clinical observation, hematoxylin-eosin staining, Massion staining, real-time PCR and immunohistochemistry for alpha -SMA were performed on days 7, 14, 21 and 30 following surgery. RESULTS: Statistical differences were noted in survival. analysis and intraocular pressure(IOP) among groups on days 7, 14, 21 and 30 following surgery. Histology, immunohistochemistry and real-time PCR further demonstrated that trabeculectomy in combination with freeze-dried bilayered fibrin-binding amniotic membrane resulted in good wound healing and no scar formation. CONCLUSION: Self-made freeze-dried bilayered fibrin-binding amniotic membrane may inhibit the formation of scarring in glaucoma after trabeculectomy.
第一作者单位：[1]Huazhong Univ Sci & Technol, Union Hosp, Dept Ophthalmol, Tongji Med Coll, Wuhan 430030, Hubei Province, Peoples R China
通讯作者：
通讯机构：[1]Huazhong Univ Sci & Technol, Union Hosp, Dept Ophthalmol, Tongji Med Coll, Wuhan 430030, Hubei Province, Peoples R China[*1]Huazhong Univ Sci & Technol, Union Hosp, Dept Ophthalmol, Tongji Med Coll, Wuhan 430022, Hubei Province, Peoples R China
推荐引用方式(GB/T 7714)：
Li Wan,Che Wen-Jian,Zhang Ming-Chang.Study of self-made freeze-dried bilayered fibrin-binding amniotic membrane in ocular trabeculectomy in rabbits[J].INTERNATIONAL JOURNAL OF OPHTHALMOLOGY.2011,4(6):582-589.doi:10.3980/j.issn.2222-3959.2011.06.02.
APA：
Li, Wan,Che, Wen-Jian&Zhang, Ming-Chang.(2011).Study of self-made freeze-dried bilayered fibrin-binding amniotic membrane in ocular trabeculectomy in rabbits.INTERNATIONAL JOURNAL OF OPHTHALMOLOGY,4,(6)
MLA：
Li, Wan,et al."Study of self-made freeze-dried bilayered fibrin-binding amniotic membrane in ocular trabeculectomy in rabbits".INTERNATIONAL JOURNAL OF OPHTHALMOLOGY 4..6(2011):582-589
