单位：[1]Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, Hubei, P.R. China.[2]Department of Orthopedics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,P.R. China华中科技大学同济医学院附属同济医院外科学系骨科[3]Department of Pathology and Pathophysiology, Medical College, Jianghan University, Wuhan, Hubei, P.R. China[4]Department of Radiology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,P.R. China.华中科技大学同济医学院附属同济医院放射科
Background: Current strategies for craniofacial defect are faced with unmet outcome. Combining 3D-printing with safe, noninvasive magnetic therapy could be a promising breakthrough. Methods: In this study, polylactic acid/hydroxyapatite (PLA/HA) composite scaffold was fabricated. After seeding rat bone marrow mesenchymal stem cells (BMSCs) on scaffolds, the effects of electromagnetic fields (EMF) on the proliferation and osteogenic differentiation capacity of BMSCs were investigated. Additionally, 6-mm critical-sized calvarial defect was created in rats. BMSC-laden scaffolds were implanted into the defects with or without EMF treatment. Results: Our results showed that PLA/HA composite scaffolds exhibited uniform porous structure, high porosity (70%), suitable compression strength (31.18 +/- 4.86 MPa), modulus of elasticity (10.12 +/- 1.24 GPa), and excellent cytocompatibility. The proliferation and osteogenic differentiation capacity of BMSCs cultured on the scaffolds were enhanced with EMF treatment. Mechanistically, EMF exposure functioned partly by activating mitogen-activated protein kinase (MAPK) or MAPK-associated ERK and JNK pathways. In vivo, significantly higher new bone formation and vascularization were observed in groups involving scaffold, BMSCs, and EMF treatment, compared to scaffold alone. Furthermore, after 12 weeks of implanting, craniums in groups including scaffold, BMSCs, and EMF exposure showed the greatest biomechanical properties. Conclusion: In conclusion, EMF treatment combined with 3D-printed scaffold has great potential applications in craniofacial regeneration.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [51537004, 51877097, 51907077]
基金编号：51537004 51877097 51907077
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|2 区医学
小类|2 区医学：研究与实验3 区细胞生物学
最新[2025]版：
大类|2 区医学
小类|2 区细胞与组织工程2 区细胞生物学2 区医学：研究与实验
JCR分区：
出版当年[2018]版：
Q1MEDICINE, RESEARCH & EXPERIMENTALQ2CELL BIOLOGY
最新[2023]版：
Q1CELL & TISSUE ENGINEERINGQ1CELL BIOLOGYQ1MEDICINE, RESEARCH & EXPERIMENTAL
第一作者单位：[1]Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, Hubei, P.R. China.[2]Department of Orthopedics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,P.R. China
通讯作者：
推荐引用方式(GB/T 7714)：
tu chang,chen jingyuan,huang chunwei,et al.Effects of electromagnetic fields treatment on rat critical-sized calvarial defects with a 3D-printed composite scaffold[J].STEM CELL RESEARCH & THERAPY.2020,11(1):doi:10.1186/s13287-020-01954-7.
APA：
tu,chang,chen,jingyuan,huang,chunwei,xiao,yifan,tang,xiangyu...&liu,chaoxu.(2020).Effects of electromagnetic fields treatment on rat critical-sized calvarial defects with a 3D-printed composite scaffold.STEM CELL RESEARCH & THERAPY,11,(1)
MLA：
tu,chang,et al."Effects of electromagnetic fields treatment on rat critical-sized calvarial defects with a 3D-printed composite scaffold".STEM CELL RESEARCH & THERAPY 11..1(2020)
医学