The use of electromagnetic fields (EMFs) to treat nonunion fractures developed from observations in the mid-1900s. Whether EMF directly regulates the bone marrow mesenchymal stem cells (MSCs), differentiating into osteoblasts or adipocytes, remains unknown. In the present study, we investigated the roles of sinusoidal FMF of 15 Hz, 1 mT in differentiation along these separate lineages using rat bone marrow MSCs. Our results showed that EMF promoted osteogenic differentiation of the stem cells and concurrently inhibited adipocyte formation. EMF increased alkaline phosphatase (ALP) activity and mineralized nodule formation, and stimulated osteoblast-specific mRNA expression of RUNX2, ALP, BMP2, DEX5, and BSP. In contrast, EMF decreased adipogenesis and inhibited adipocyte-specific mRNA expression of adipsin, AP-2, and PPAR gamma 2, and also inhibited protein expression of PPAR gamma 2. These observations suggest that commitment of MSCs into osteogenic or adipogenic lineages is influenced by EMF. Bioelectromagneties 31:277-285, 2010. (C) 2009 Wiley-Liss. Inc.
基金：
National Natural Sciences Foundation of China [50477043]
第一作者单位：[1]Huazhong Univ Sci & Technol,Dept Orthoped,Tongji Hosp,Coll Med,Wuhan 430074,Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Yang Yong,Tao Chaoxiong,Zhao Dongming,et al.EMF Acts on Rat Bone Marrow Mesenchymal Stem Cells to Promote Differentiation to Osteoblasts and to Inhibit Differentiation to Adipocytes[J].BIOELECTROMAGNETICS.2010,31(4):277-285.doi:10.1002/bem.20560.
APA：
Yang, Yong,Tao, Chaoxiong,Zhao, Dongming,Li, Feng,Zhao, Wenchun&Wu, Hua.(2010).EMF Acts on Rat Bone Marrow Mesenchymal Stem Cells to Promote Differentiation to Osteoblasts and to Inhibit Differentiation to Adipocytes.BIOELECTROMAGNETICS,31,(4)
MLA：
Yang, Yong,et al."EMF Acts on Rat Bone Marrow Mesenchymal Stem Cells to Promote Differentiation to Osteoblasts and to Inhibit Differentiation to Adipocytes".BIOELECTROMAGNETICS 31..4(2010):277-285
