Objective: This study is to investigate the role and mechanism of miRNA-204 in human cervical carcinoma. Methods: 73 cases of cervical carcinoma patients diagnosed and operated in our hospital from February 2014 to August 2014 were enrolled. And, 85 aged-matched healthy volunteers were set as controls. Tumor tissues, the corresponding tumor-adjacent tissues and blood specimens were collected. qRT-PCR was applied for detecting expression levels of Blc-2 mRNA and miR-204, Western Blot and ELISA were applied for detecting protein level of Bcl-2 in tissues, SiHa cells and serum, respectively. Luciferase reporter assay was applied to investigate whether miR-204 could regulate the expression of Bcl-2 by direct binding to the 3'UTR. Flow cytometry was applied to evaluate the cell apoptosis rate of miR-204 mimics and siRNA transfected SiHa cells. Results: The expressions of miRNA-204 were significantly down-regulated in tumor tissues and blood in cervical carcinoma patients, while Bcl-2 mRNA and protein were significantly up-regulated accordingly. Overexpression of miRNA-204 resulted in significantly downregulated expression of Bcl-2, and increased apoptosis rate of SiHa cells. Luciferase reporter assay showed that miRNA-204 regulated the expression of Bcl-2 by binding to the 3'-UTR. Conclusion: The expression of Bcl-2 was significantly up-regulated in tumor tissues and blood in cervical cancer patients, and this increase may be related to the down-regulated expression of miRNA-204. Our data suggests that miRNA-204 may regulate cell apoptosis and expression of related proteins in cervical carcinoma cells through Bcl-2.