Natural biominerals are formed through metastable amorphous precursor phases via a bottom-up, nanoparticle-mediated mineralization mechanism. Using an acid-etched human dentin model to create a layer of completely demineralized collagen matrix, a bio-inspired mineralization scheme has been developed based on the use of dual biomimetic analogs. These analogs help to sequester fluidic amorphous calcium phosphate nanoprecursors and function as templates for guiding homogeneous apatite nucleation within the collagen fibrils. By adopting this scheme for remineralizing adhesive resin-bonded, completely demineralized dentin, we have been able to redeposit intrafibrillar and extrafibrillar apatites in completely demineralized collagen matrices that are imperfectly infiltrated by resins. This study utilizes a spectrum of completely and partially demineralized dentin collagen matrices to further validate the necessity for using a biomimetic analog-containing medium for remineralizing resin-infiltrated partially demineralized collagen matrices in which remnant seed crystallites are present. In control specimens in which biomimetic analogs are absent from the remineralization medium, remineralization could only be seen in partially demineralized collagen matrices, probably by epitaxial growth via a top-down crystallization approach. Conversely, in the presence of biomimetic analogs in the remineralization medium, intrafibrillar remineralization of completely demineralized collagen matrices via a bottom-up crystallization mechanism can additionally be identified. The latter is characterized by the transition of intrafibrillar minerals from an inchoate state of continuously braided microfibrillar electron-dense amorphous strands to discrete nanocrystals, and ultimately into larger crystalline platelets within the collagen fibrils. Biomimetic remineralization via dual biomimetic analogs has the potential to be translated into a functional delivery system for salvaging failing resin-dentin bonds. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
基金：
National Institute of Dental and Craniofacial Research [R21 DE019213-01]
第一作者单位：[2]Kyung Hee Univ, Sch Dent, Dept Conservat Dent, Seoul 130701, South Korea
通讯作者：
通讯机构：[1]Med Coll Georgia, Sch Dent, Dept Oral Biol, Augusta, GA 30912 USA[*1]Med Coll Georgia, Sch Dent, Dept Oral Biol, 1120,15th St,CL 2120, Augusta, GA 30912 USA
推荐引用方式(GB/T 7714)：
Kim Jongryul,Arola Dwayne D.,Gu Lisha,et al.Functional biomimetic analogs help remineralize apatite-depleted demineralized resin-infiltrated dentin via a bottom-up approach[J].ACTA BIOMATERIALIA.2010,6(7):2740-2750.doi:10.1016/j.actbio.2009.12.052.
APA：
Kim, Jongryul,Arola, Dwayne D.,Gu, Lisha,Kim, Young Kyung,Mai, Sui...&Tay, Franklin R..(2010).Functional biomimetic analogs help remineralize apatite-depleted demineralized resin-infiltrated dentin via a bottom-up approach.ACTA BIOMATERIALIA,6,(7)
MLA：
Kim, Jongryul,et al."Functional biomimetic analogs help remineralize apatite-depleted demineralized resin-infiltrated dentin via a bottom-up approach".ACTA BIOMATERIALIA 6..7(2010):2740-2750
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