单位：[1]Xiamen Univ, Natl Inst Diagnost & Vaccine Dev Infect Dis, Sch Life Sci, Xiamen, Peoples R China[2]Capital Med Univ, Youan Hosp, Beijing, Peoples R China[3]Fourth Mil Med Univ, Xijing Hosp, Xian 710032, Peoples R China[4]Huazhong Univ Sci & Technol, Tongji Hosp, Wuhan 430074, Peoples R China华中科技大学同济医学院附属同济医院[5]Ctr Dis Control & Prevent Xiamen, Xiamen, Peoples R China[6]Fujian Med Univ, Quanzhou Hosp 1, Quanzhou, Peoples R China[7]Xiamen Blood Ctr, Xiamen, Peoples R China
A novel immunoassay that detects simultaneously hepatitis B virus (HBV) PreS1 and/or core-related antigens was developed and evaluated for its potential for detecting hepatitis B surface antigen (HBsAg) variants. The detection limits of the assay was 10(2.9 +/- 05) copies/mL (mean +/- SD) for HBsAg-positive sera with different genotypes, and 10(3.5 +/- 1.2) copies/mL for HBsAg variants sera. The specificity of the assay was 99.9% (95% Cl: 99.7-99.9%, 4551 healthy individuals). The sensitivities were 93.9% (95% Cl: 92.8-94.9%), 59.3% (95% Cl: 38.7-77.6%) and 80% (95% CI: 44.4-97.5%) in three independent groups which include: 2065 hepatitis patients, 27 patients with occult hepatitis B and 10 HBsAg variants, respectively. In addition, a novel premature stop code mutation at position 112 of HBsAg was observed in two patients with chronic hepatitis B with different genotypes. (C) 2010 Elsevier B.V. All rights reserved.
基金：
National Advanced Technology Research and Development Program [2006AA02Z442, 2006AA020905]; Program for New Century Excellent Talents in University [NCET-05-0567]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2009]版：
大类|4 区医学
小类|4 区生化研究方法4 区生物工程与应用微生物4 区病毒学
最新[2025]版：
大类|4 区医学
小类|4 区生化研究方法4 区生物工程与应用微生物4 区病毒学
JCR分区：
出版当年[2008]版：
Q2BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3BIOCHEMICAL RESEARCH METHODSQ3VIROLOGY
最新[2023]版：
Q3BIOCHEMICAL RESEARCH METHODSQ3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3VIROLOGY
第一作者单位：[1]Xiamen Univ, Natl Inst Diagnost & Vaccine Dev Infect Dis, Sch Life Sci, Xiamen, Peoples R China
通讯作者：
通讯机构：[1]Xiamen Univ, Natl Inst Diagnost & Vaccine Dev Infect Dis, Sch Life Sci, Xiamen, Peoples R China[*1]Xiamen Univ, Natl Inst Diagnost & Vaccine Dev Infect Dis, Sch Life Sci, Simin Nan Lu 422, Xiamen, Peoples R China
推荐引用方式(GB/T 7714)：
Yuan Quan,Ge Shengxiang,Xiong Junhui,et al.A novel immunoassay for PreS1 and/or core-related antigens for detection of HBsAg variants[J].JOURNAL OF VIROLOGICAL METHODS.2010,168(1-2):108-113.doi:10.1016/j.jviromet.2010.04.029.
APA：
Yuan, Quan,Ge, Shengxiang,Xiong, Junhui,Yan, Qiang,Li, Zhuo...&Xia, Ningshao.(2010).A novel immunoassay for PreS1 and/or core-related antigens for detection of HBsAg variants.JOURNAL OF VIROLOGICAL METHODS,168,(1-2)
MLA：
Yuan, Quan,et al."A novel immunoassay for PreS1 and/or core-related antigens for detection of HBsAg variants".JOURNAL OF VIROLOGICAL METHODS 168..1-2(2010):108-113
