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Rapid and selective determination of urinary lysozyme based on magnetic molecularly imprinted polymers extraction followed by chemiluminescence detection

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单位: [1]Huazhong Univ Sci & Technol, MOE Key Lab Environm & Hlth, Inst Environm Med, Sch Publ Hlth,Tongji Med Coll, Wuhan 430030, Hubei, Peoples R China [2]Huazhong Univ Sci & Technol, Dept Clin Lab, Tongji Hosp, Tongji Med Coll, Wuhan 430030, Hubei, Peoples R China [3]Changwon Natl Univ, Dept Chem, Chang Won 641773, South Korea
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关键词: Molecularly imprinted polymers Magnetic separation Chemiluminescence Lysozyme Urine samples

摘要:
A rapid, low cost and selective chemiluminescence method coupled with magnetic molecularly imprinted polymers extraction was developed to detect lysozyme in human urine samples. Compared with traditional solid-phase extraction, this method could achieve selective extraction for the lysozyme, avoid the time consuming elution from a column or centrifugation steps, and then showed great potential in the high-throughput screening of clinical samples. The parameters affecting the performance of extraction and chemiluminescence were investigated. Under optimal conditions, the whole analytical procedure was completed within 12 min and spiked recovery ranged from 90.1% to 103.7% (R.S.D. <= 6.7%). The limit of quantitation was 5 ng m L-1. Furthermore, the results obtained by the proposed method were linearly correlated to those by commercial lysozyme detection kit (r = 0.9595). Finally, the validated method was used to measure the urinary lysozyme of renal disease patients and healthy controls. The results confirmed the reliability and practicality of the protocol and revealed a good perspective of this method for biological sample analysis. (C) 2011 Elsevier B.V. All rights reserved.

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出版当年[2010]版:
大类 | 3 区 化学
小类 | 2 区 分析化学
最新[2025]版:
大类 | 2 区 化学
小类 | 2 区 分析化学
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出版当年[2009]版:
Q1 CHEMISTRY, ANALYTICAL
最新[2024]版:
Q1 CHEMISTRY, ANALYTICAL

影响因子: 最新[2024版] 最新五年平均 出版当年[2009版] 出版当年五年平均 出版前一年[2008版] 出版后一年[2010版]

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第一作者单位: [1]Huazhong Univ Sci & Technol, MOE Key Lab Environm & Hlth, Inst Environm Med, Sch Publ Hlth,Tongji Med Coll, Wuhan 430030, Hubei, Peoples R China
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通讯机构: [1]Huazhong Univ Sci & Technol, MOE Key Lab Environm & Hlth, Inst Environm Med, Sch Publ Hlth,Tongji Med Coll, Wuhan 430030, Hubei, Peoples R China [*1]Huazhong Univ Sci & Technol, MOE Key Lab Environm & Hlth, Inst Environm Med, Sch Publ Hlth,Tongji Med Coll, 13 Hangkong Rd, Wuhan 430030, Hubei, Peoples R China
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