Cytotoxic T lymphocyte antigen 4 (CTLA-4) is an inhibitory T cell receptor predominately expressed on activated T cells and plays an important role in regulation of specific T cell responses to viral infection. The woodchuck model is an informative animal model for hepatitis B virus (HBV) infection. In this study, the extracellular region of woodchuck CTLA-4 (wCTLA-4) was cloned and the fusion protein of GST-wCTLA-4 was expressed and purified. Polyclonal antibody against GST-wCTLA-4 (anti-GST-wCTLA-4) was prepared. The full length wCTLA-4 protein expressed in transfected baby hamster kidney cells was detected by anti-GST-wCTLA-4 in western blot analysis and immunofluorescence staining. Anti-GST-wCTLA-4 provides a useful tool to study the role of CTLA-4 in T-cell response in the woodchuck model. Further, the blocking of CTLA-4 with anti-GST-wCTLA-4, as a novel therapy approach for chronic hepatitis B virus infection, could be studied in woodchuck model now. (C) 2011 Elsevier Inc. All rights reserved.
基金：
Natural Science Foundation of Hubei Province [2009-59]; Fundamental Research Funds for the Central Universities [HUST: 2010M5119]; Deutsche Forschungsgemeinschaft [GRK1045/2, Transregio TRR60]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2011]版：
大类|4 区生物
小类|4 区生化研究方法4 区生化与分子生物学4 区生物工程与应用微生物
最新[2025]版：
大类|4 区生物学
小类|4 区生化研究方法4 区生化与分子生物学4 区生物工程与应用微生物
JCR分区：
出版当年[2010]版：
Q3BIOCHEMISTRY & MOLECULAR BIOLOGYQ3BIOCHEMICAL RESEARCH METHODSQ3BIOTECHNOLOGY & APPLIED MICROBIOLOGY
最新[2023]版：
Q4BIOCHEMICAL RESEARCH METHODSQ4BIOCHEMISTRY & MOLECULAR BIOLOGYQ4BIOTECHNOLOGY & APPLIED MICROBIOLOGY
第一作者单位：[1]Huazhong Univ Sci & Technol, Dept Microbiol, Tongji Med Coll, Wuhan 430030, Peoples R China
通讯作者：
通讯机构：[1]Huazhong Univ Sci & Technol, Dept Microbiol, Tongji Med Coll, Wuhan 430030, Peoples R China[*1]Huazhong Univ Sci & Technol, Dept Microbiol, Tongji Med Coll, Hangkong Rd 13, Wuhan 430030, Peoples R China
推荐引用方式(GB/T 7714)：
Yang Yinke,Wang Baoju,Yang Dongliang,et al.Prokaryotic expression of woodchuck cytotoxic T lymphocyte antigen 4 (wCTLA-4) and preparation of polyclonal antibody to wCTLA-4[J].PROTEIN EXPRESSION AND PURIFICATION.2012,81(2):181-185.doi:10.1016/j.pep.2011.10.007.
APA：
Yang, Yinke,Wang, Baoju,Yang, Dongliang,Lu, Mengji&Xu, Yang.(2012).Prokaryotic expression of woodchuck cytotoxic T lymphocyte antigen 4 (wCTLA-4) and preparation of polyclonal antibody to wCTLA-4.PROTEIN EXPRESSION AND PURIFICATION,81,(2)
MLA：
Yang, Yinke,et al."Prokaryotic expression of woodchuck cytotoxic T lymphocyte antigen 4 (wCTLA-4) and preparation of polyclonal antibody to wCTLA-4".PROTEIN EXPRESSION AND PURIFICATION 81..2(2012):181-185
