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Anti-arthritic effects of FasL gene transferred intra-articularly by an inducible lentiviral vector containing improved tet-on system

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单位： [1]Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Anesthesiol, Hangzhou 310009, Zhejiang, Peoples R China [2]Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Anesthesiol, Wuhan 430030, Peoples R China

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关键词： Inducible lentiviral vector FasL gene Doxycycline Rheumatoid arthritis

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The objective of this study is to construct and identify an inducible lentiviral vector containing improved tet-on system and FasL gene and observe its effects on pristane-induced arthritis (PIA). FasL gene was amplified from the spleen of Lewis rats by RT-PCR. The tet-on system was improved with insertion of a chicken chromatin insulator (cHS4) element and an rtTA-dependent, tet-responsive element containing modifications of the tetO sequence (TRE-tight1). Pro-apoptosis effect of the vector pTREFasLcHS4V16 on synovial cells was evaluated by flow cytometer in vitro. Anti-arthritis effects of the vector on PIA after intra-articular injection were observed by clinical evaluation and joint histology. Cytokines in synovial tissue were measured by ELISA. The recombinant inducible lentiviral vector pTREFasLcHS4V16 was successfully constructed. The expression response and the pro-apoptosis effects of the vector were doxycycline dose-dependent. The vector injected intra-articularly attenuated the severity of PIA and decreased the level of cytokines in inflamed joints. pTREFasLcHS4V16 with an improved tet-on system can precisely regulate the expression of FasL gene and apoptosis. Anti-arthritis effects were observed after intra-articular injection of the inducible vector.

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出版当年[2013]版：

大类 | 4 区医学

小类 | 4 区风湿病学

最新[2025]版：

大类 | 3 区医学

小类 | 4 区风湿病学

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出版当年[2012]版：

Q3 RHEUMATOLOGY

最新[2023]版：

Q2 RHEUMATOLOGY

影响因子： 3.2 最新[2023版] 2.9 最新五年平均 2.214 出版当年[2012版] 1.866 出版当年五年平均 1.885 出版前一年[2011版] 1.627 出版后一年[2013版]

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第一作者单位： [1]Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Anesthesiol, Hangzhou 310009, Zhejiang, Peoples R China

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