The immunoaffinity of protein with ligand is broadly involved in many bioanalytical methods. Affinity-ultrafiltration mass spectrometry (AUF-MS), a platform based on interaction of protein-ligand affinity, has been developed to fish out interesting molecules from complex matrixes. Here we reviewed the basics of AUF=MS and its recent applications to pharmaceutical field, i.e.-target-oriented discovery-of-lead compounds from combinatorial libraries and natural product extracts, and determination of free drug concentration in biosamples. Selected practical examples were highlighted to illustrate the advances of AUF-MS in pharmaceutical fields. The future prospects were also presented. (C) 2016 Elsevier B.V. All rights reserved.
第一作者单位：[1]Zhengzhou Univ, Affiliated Hosp 1, Dept Pharm, Zhengzhou 450052, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Wei Han,Zhang Xiaojian,Tian Xin,et al.Pharmaceutical applications of affinity-ultrafiltration mass spectrometry: Recent advances and future prospects[J].JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS.2016,131:444-453.doi:10.1016/j.jpba.2016.09.021.
APA：
Wei, Han,Zhang, Xiaojian,Tian, Xin&Wu, Guanghua.(2016).Pharmaceutical applications of affinity-ultrafiltration mass spectrometry: Recent advances and future prospects.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,131,
MLA：
Wei, Han,et al."Pharmaceutical applications of affinity-ultrafiltration mass spectrometry: Recent advances and future prospects".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 131.(2016):444-453
