The aim of the study was to determine the expression profiles of message RNAs and long non-coding RNAs in CD4(+)ET cells of asthmatic patients and to explore the clinical value and biological function. Expression profiles in CD4(+)T cells of asthmatic patients and healthy controls were analyzed by micro array. We found 2725 lncRNAs and 3167 mRNA5 differentially expressed. The data were validated by quantitative real time polymerase chain reaction, with 3 up-regulated (ENST00000444682, ENST00000566098, ENST00000583179) and 1 down-regulated (ENST00000579468) IncRNAs found. Receiver operating characteristic curve analysis showed the area under the curve was 0.7058, 0.9026, 0.8361, 0.8316, respectively. Spearman correlation analysis showed that ENST00000566098 was positively related with IL-13 and ENST00000579468 was positively related with peak expiratory flow. Bioinformatics analyses were performed to explore the function of lncRNAs. Specific lncRNAs aberrantly expressed in CD4(+)T cells may take part in the development of asthma and may be used as biomarkers for diagnosis. (C) 2018 Published by Elsevier Inc.
基金：
National Natural Science Fund of China [81570024, 91742111, 81470226]
第一作者单位：[1]Huazhong Univ Sci & Technol,Dept Resp & Crit Care Med,Key Cite Natl Clin Res Ctr Resp Dis,Key Lab Pulm Dis,Hlth Minist,Tongji Hosp,Tongji M,Wuhan,Hubei,Peoples R China
通讯作者：
通讯机构：[1]Huazhong Univ Sci & Technol,Dept Resp & Crit Care Med,Key Cite Natl Clin Res Ctr Resp Dis,Key Lab Pulm Dis,Hlth Minist,Tongji Hosp,Tongji M,Wuhan,Hubei,Peoples R China[*1]Huazhong Univ Sci & Technol,Tongji Hosp,Tongji Med Coll,Dept Resp Dis,1095 Jiefang Dadao Rd,Wuhan 430030,Hubei,Peoples R China
推荐引用方式(GB/T 7714)：
Qi Xuefei,Chen Huilong,Huang Zhenli,et al.Aberrantly expressed lncRNAs identified by microarray analysis in CD4+T cells in asthmatic patients[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2018,503(3):1557-1562.doi:10.1016/j.bbrc.2018.07.079.
APA：
Qi, Xuefei,Chen, Huilong,Huang, Zhenli,Fu, Bohua,Wang, Yi...&Xiong, Weining.(2018).Aberrantly expressed lncRNAs identified by microarray analysis in CD4+T cells in asthmatic patients.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,503,(3)
MLA：
Qi, Xuefei,et al."Aberrantly expressed lncRNAs identified by microarray analysis in CD4+T cells in asthmatic patients".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 503..3(2018):1557-1562
