单位：[1]Department of Obstetrics and Gynecology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,People’s Republic of China华中科技大学同济医学院附属同济医院妇产科教研室妇产科学系[2]Department of Pharmacology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,People’s Republic of China华中科技大学同济医学院附属同济医院药学部
Purpose: In this study, we investigated the effect of Realgar on the apoptosis of HPV 16-positive cervical cells in vitro. Methods: The effect of Realgar on the apoptosis of HPV16-positive cervical cells was investigated by annexin V-fluorescein isothiocyanate/propidium iodide staining and growth inhibition assays using HPV16-positive cervical cancer cell line SiHa and HPV16-positive immortalized cervical epithelial cell line S12. The expression of genes was measured by real-time PCR, and the expression of corresponding proteins was detected by Western blotting. The adhesion and invasion of cells were detected by adhesion assay and Transwell invasion assay, respectively. Results: The Realgar inhibited the proliferation and induced the apoptosis of SiHa and S12 cells in a dose- and time-dependent manner. The Realgar suppressed the expression of HPV16 E7 and caspase-3. The Realgar suppressed the adhesion and invasion of both cells. Conclusion: The Realgar induced apoptosis, inhibited the proliferation of HPV16-positive cell lines through a HPV16 E7-dependent pathway, and inhibited cell adhesion and invasion.
基金：
National Natural Science Funding of ChinaNational Natural Science Foundation of China (NSFC) [81403166, 81502252]
第一作者单位：[1]Department of Obstetrics and Gynecology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,People’s Republic of China
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通讯作者：
推荐引用方式(GB/T 7714)：
ding wencheng,ji teng,xiong wei,et al.Realgar, a traditional Chinese medicine, induces apoptosis of HPV16-positive cervical cells through a HPV16 E7-related pathway[J].DRUG DESIGN DEVELOPMENT AND THERAPY.2018,12:3459-3469.doi:10.2147/DDDT.S172525.
APA：
ding,wencheng,ji,teng,xiong,wei,li,tian,pu,demin&liu,rong.(2018).Realgar, a traditional Chinese medicine, induces apoptosis of HPV16-positive cervical cells through a HPV16 E7-related pathway.DRUG DESIGN DEVELOPMENT AND THERAPY,12,
MLA：
ding,wencheng,et al."Realgar, a traditional Chinese medicine, induces apoptosis of HPV16-positive cervical cells through a HPV16 E7-related pathway".DRUG DESIGN DEVELOPMENT AND THERAPY 12.(2018):3459-3469
