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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric identification and antifungal susceptibility analysis of Candida species isolated from patients with invasive yeast infections in five university hospitals

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单位: [1]Huazhong Univ Sci & Technol, Wuhan Hosp 1, Tongji Med Coll, Dept Clin Microbiol Lab, 215 Zhongshan Ave, Wuhan 430022, Hubei, Peoples R China [2]PLA 302 Hosp, Dept Clin Lab, 100 West Fourth Ring Rd, Beijing 100166, Peoples R China [3]Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Clin Microbiol Lab, 1095 Liberation Ave, Wuhan 430030, Hubei, Peoples R China [4]Hubei Women & Childrens Hosp, Dept Clin Microbiol Lab, 745 Wuluo Rd, Wuhan 430070, Hubei, Peoples R China [5]Wuhan Women & Children Med Care Ctr, Dept Clin Lab, 100 Xianggang Rd, Wuhan 430015, Hubei, Peoples R China [6]Wuhan Univ, Zhongnan Hosp, Dept Clin Microbiol Lab, 169 Donghu Rd, Wuhan 430071, Hubei, Peoples R China [7]Soochow Univ, Affiliated Hosp 1, Dept Clin Microbiol Lab, 188 Shizi St, Suzhou 215006, Peoples R China
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关键词: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry Candida Yeast Identification Antifungal susceptibility test

摘要:
In this multicenter study, we compared the performance of the Bruker Biotyper MS system and VITEK 2 YST systems for invasive yeast identification, investigated the distribution of isolated species, and evaluated the antifungal susceptibility profiles of Candida albicans, Candida parapsilosis, and Candida tropicalis. In cases of discrepant results lack of identification with either method, molecular identification techniques were employed. We tested 216 clinical isolates, and concordance between the two methods was observed for 192/216 isolates (88.9%). For five unidentified strains (2.3%), an internal transcribed spacer (ITS) sequencing approach was used. In brief, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) provided short turnaround times and more reliable results than those of Vitek 2 YST. In Wuhan, C. albicans, C. parapsilosis, Candida glabrata, and C. tropicalis were the most common pathogens (93.0%) in patients with candidemia. Cryptococcus neoformans was mainly detected in cerebrospinal fluid samples (88.9%). Trichosporon asahii were all isolated from drainage fluids in the Surgery. Candida albicans was clearly susceptible to azoles, while C. parapsilosis and C. tropicalis displayed differences in susceptibility to azoles. Our findings provide a basis for the practical application of MALDI-ToF MS for identification and for the use of ATB FUNGUS 3 to characterize the susceptibility of Candida spp., thereby providing significant data for therapeutic decisions.

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出版当年[2018]版:
大类 | 4 区 生物
小类 | 4 区 微生物学
最新[2025]版:
大类 | 4 区 生物学
小类 | 4 区 微生物学
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出版当年[2017]版:
Q3 MICROBIOLOGY
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Q3 MICROBIOLOGY

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第一作者单位: [1]Huazhong Univ Sci & Technol, Wuhan Hosp 1, Tongji Med Coll, Dept Clin Microbiol Lab, 215 Zhongshan Ave, Wuhan 430022, Hubei, Peoples R China
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