High fat-induced inflammation in vascular endothelium can be improved by Abelmoschus esculentus and metformin via increasing the expressions of miR-146a and miR-155
Background Obesity is associated with chronic inflammation, which contributes to cardiovascular diseases. MicroRNAs (miRNAs) are reported to be involved in vascular inflammation and atherosclerosis. Abelmoschus esculentus (AE) and metformin have been suggested to improve inflammation in vascular system. The aim of this study is to evaluate whether miRNAs are involved in high fat induced endothelial inflammation, and whether AE and metformin improve endothelial inflammation by regulating miRNAs. Methods We established high fat treated rats and human aortic endothelial cells (HAECs). AE and metformin were added to explore their effects on endothelial inflammation induced by high fat and the possible mechanism. Results The vascular inflammatory genes were increased in rats treated with high fat diet. The decreased miR-146a and miR-155 were involved in endothelial inflammation induced by high fat through targeting IL-1 receptor-associated kinase 1 (IRAK1), TNF receptor-associated factor 6 (TRAF6) and nuclear factor-kappa B p65 (NF-kappa B p65), respectively. While AE and metformin could ameliorate the endothelial inflammation by increasing miR-146a and miR-155. Conclusions These results indicate that miR-146a and miR-155 play roles in the high fat induced endothelial inflammation, which could be potential therapeutic targets. AE and metformin can attenuate endothelial inflammation through regulating miR-146a and miR-155.
基金：
National Natural Science Foundation of China [81600639, 81471069, 81770843]; Knowledge Innovation Project of Hubei Province [2016CFA025]
第一作者单位：[1]Huazhong Univ Sci & Technol, Dept Endocrinol, Union Hosp, Tongji Med Coll, Wuhan 430022, Peoples R China[2]Huazhong Univ Sci & Technol, Dept Endocrinol, Tongji Hosp, Tongji Med Coll, Wuhan 430030, Peoples R China
通讯作者：
通讯机构：[1]Huazhong Univ Sci & Technol, Dept Endocrinol, Union Hosp, Tongji Med Coll, Wuhan 430022, Peoples R China[3]Hubei Prov Clin Res Ctr Diabet & Metab Disorders, Wuhan, Peoples R China
推荐引用方式(GB/T 7714)：
Gou Luoning,Liu Geng,Ma Rong,et al.High fat-induced inflammation in vascular endothelium can be improved by Abelmoschus esculentus and metformin via increasing the expressions of miR-146a and miR-155[J].NUTRITION & METABOLISM.2020,17(1):doi:10.1186/s12986-020-00459-7.
APA：
Gou, Luoning,Liu, Geng,Ma, Rong,Regmi, Anita,Zeng, Tianshu...&Chen, Lulu.(2020).High fat-induced inflammation in vascular endothelium can be improved by Abelmoschus esculentus and metformin via increasing the expressions of miR-146a and miR-155.NUTRITION & METABOLISM,17,(1)
MLA：
Gou, Luoning,et al."High fat-induced inflammation in vascular endothelium can be improved by Abelmoschus esculentus and metformin via increasing the expressions of miR-146a and miR-155".NUTRITION & METABOLISM 17..1(2020)
