单位：[a]School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China[b]Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang, 325001, China[c]The First Affiliated Hospital, Department of Preventive Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China[d]Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China华中科技大学同济医学院附属同济医院检验科
Exosomes, derived from various biofluids, may serve as potential biomarkers for cancer early detection. Nevertheless, exosome clinical translation remains challenging due to the lack of reliable isolation and detection methods. Herein, we present a novel integrated microfluidic device specifically designed for isolation and in-situ detection of lung cancer-specific exosomes collected from patient's urine. The new device has been fabricated using polymethyl methacrylate (PMMA) and a nanoporous gold (Au) nanocluster membrane modified with the capture antibody. The second antibody-conjugated Au nanorod probe was then loaded to identify and quantify lung cancer-specific exosomes using a dark field microscope. AuNC-Exosome-AuR complex produces a significant scattering wavelength shift and an improved scattering intensity due to resonance Rayleigh scattering, which enables the ultrasensitive detection of exosomes with a LOD below 1000 particles/mL. The proteomic analysis revealed that the high-purity exosomes has been isolated by the device. We then validated this method with 500 mu L urine samples from lung cancer patients and controls, which showed great promise for differentiating early-stage lung cancer patients from healthy individuals. Taken together, the presented method is fast and ultrasensitive and can be easily adapted for the isolation and detection of cancer specific exosomes from other malignant tumors.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31741036, 21904098, 81572071]; Zhejiang Provincial [WKJ-ZJ-1910]; Ministry of Health Research Fund for Medical Sciences [WKJ-ZJ-1910]; Wenzhou Medical University [89218012, 89219012]; Wenzhou Institute, University of Chinese Academy of Sciences [WIBEZD2017006-05]
第一作者单位：[a]School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China[b]Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang, 325001, China
共同第一作者：
通讯作者：
通讯机构：[a]School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China[b]Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang, 325001, China[c]The First Affiliated Hospital, Department of Preventive Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China[*1]School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
推荐引用方式(GB/T 7714)：
Qinsi Yang,Liming Cheng,Liang Hu,et al.An integrative microfluidic device for isolation and ultrasensitive detection of lung cancer-specific exosomes from patient urine[J].BIOSENSORS & BIOELECTRONICS.2020,163:doi:10.1016/j.bios.2020.112290.
APA：
Qinsi Yang,Liming Cheng,Liang Hu,Doudou Lou,Ting Zhang...&Fei Liu.(2020).An integrative microfluidic device for isolation and ultrasensitive detection of lung cancer-specific exosomes from patient urine.BIOSENSORS & BIOELECTRONICS,163,
MLA：
Qinsi Yang,et al."An integrative microfluidic device for isolation and ultrasensitive detection of lung cancer-specific exosomes from patient urine".BIOSENSORS & BIOELECTRONICS 163.(2020)
工程技术