单位：[1]National “111” Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan, 430068, China [2] Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing, 100048, China [3] Department of Thoracic Surgery, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China 华中科技大学同济医学院附属同济医院胸外科[4] Zhejiang Skyherb Biotechnology Inc., Huzhou, 313300, China
The effect of green tea polysaccharide conjugate (gTPC) on the growth of E. coli was evaluated. gTPC has optimal antibacterial concentrations for E. coli DH5 alpha (4.00 mg/mL) and 25,922 (1.00 mg/mL), with the antibacterial rates of 55.75 % and 50.65 % respectively. The results of scanning electron microscopy (SEM) and transmission electron microscope (TEM) indicated that gTPC-treated E. coli cells showed obvious morphological changes, part of the cell wall became blurred, and the leakage of cytoplasmic content were observed. The proportion of the propidium iodide (PI) permeable E. coli cells treated using 0.50-2.00 mg/mL gTPC was between 8.79 %-9.49 % as well as 14.10 % (4.00 mg/mL gTPC) and 15.10 % (6.00 mg/mL gTPC). Reactive oxygen species (ROS) production of 1.00-4.00 mg/mL gTPC treated E. coli increased by 26.57 %-38.19 % with a significant increase level (p < 0.01). The outer membrane disruption played a key role in the antibacterial activity of gTPC against E. coli. This research result will open a new application field of tea polysaccharide conjugates.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31871813]; Beijing Advanced Innovation Center for Food Nutrition and Human Health
第一作者单位：[1]National “111” Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan, 430068, China
共同第一作者：
通讯作者：
通讯机构：[1]National “111” Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan, 430068, China [2] Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing, 100048, China
推荐引用方式(GB/T 7714)：
Yin Zhou,Qifeng Yao,Tong Zhang,et al.Antibacterial activity and mechanism of green tea polysaccharide conjugates against Escherichia coli[J].INDUSTRIAL CROPS AND PRODUCTS.2020,152:doi:10.1016/j.indcrop.2020.112464.
APA：
Yin Zhou,Qifeng Yao,Tong Zhang,Xiaoqiang Chen,Zhengqi Wu...&Yong Cheng.(2020).Antibacterial activity and mechanism of green tea polysaccharide conjugates against Escherichia coli.INDUSTRIAL CROPS AND PRODUCTS,152,
MLA：
Yin Zhou,et al."Antibacterial activity and mechanism of green tea polysaccharide conjugates against Escherichia coli".INDUSTRIAL CROPS AND PRODUCTS 152.(2020)
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