单位：[1]Institute of Integrated Traditional Chinese and Western Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan430030,PR China华中科技大学同济医学院附属同济医院中西医结合研究所[2]Huanggang Traditional Chinese Medicine Hospital，Hubei University of Chinese Medicine，Huanggang438000, PR China.
Ethnopharmacological relevance: Jiao-tai-wan is a well-known traditional Chinese herbal medicine formula that is used to treat insomnia and systemic inflammation. Studies indicate chronic insomnia might contribute to the prevalence of cognitive impairment. The role of systemic inflammation and intestinal permeability in the progression of neurodegenerative diseases attracts much attention. Aim of the study: This study aimed to investigate if Jiao-tai-wan plays a role in promoting the repair of the intestinal epithelial barrier to suppress systemic inflammation and cognitive impairment in sleep-deprived (SD) rats. Materials and methods: Male obesity-resistant SD rats were partially sleep-deprived for 16 weeks. During the last 8 weeks, they were treated with Jiao-tai-wan. A Morris water maze was used to analyze their cognitive ability. A beta 42 and proinflammation cytokines in the cerebrospinal fluid, tissue, or serum were determined using enzyme-linked immunosorbent assay or polymerase chain reaction. Intestinal permeability was detected using the fluorescein isothiocyanate-dextran perfusion assay method, Plasma lipopolysaccharide (LPS) levels were detected with Tachypleus Amebocyte Lysate. Western bolt was used in the signaling pathway analysis. Results: Sleep deprivation deteriorated the performance of rats in the Morris water maze and increased the A beta 42, caspase3, IL-6, and TNF-alpha levels in their brains. The intestinal TLR4/NF-kappa B pathway was activated with an increase in the expression of IL-6 and TNF-alpha. The expression of tight junction proteins was also decreased in the intestinal tissue. This increased the intestinal permeability and circulation of LPS, LPS binding protein, IL-6, and TNF-alpha. Treatment with Jiao-tai-wan could partly reverse these changes. Conclusion: Jiao-tai-wan has the potential to attenuate systemic inflammation and cognitive impairment in partially sleep-deprived rats. The possible underlying mechanism is by preventing an inflammation trigger being transferred through the gut-brain-axis.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81874382]
第一作者单位：[1]Institute of Integrated Traditional Chinese and Western Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan430030,PR China
共同第一作者：
通讯作者：
通讯机构：[1]Institute of Integrated Traditional Chinese and Western Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan430030,PR China
推荐引用方式(GB/T 7714)：
su hao,zhang chu,zou xin,et al.Jiao-tai-wan inhibits inflammation of the gut-brain-axis and attenuates cognitive impairment in insomnic rats[J].JOURNAL OF ETHNOPHARMACOLOGY.2020,250:doi:10.1016/j.jep.2019.112478.
APA：
su,hao,zhang,chu,zou,xin,lu,fuer,zeng,yong...&dong,hui.(2020).Jiao-tai-wan inhibits inflammation of the gut-brain-axis and attenuates cognitive impairment in insomnic rats.JOURNAL OF ETHNOPHARMACOLOGY,250,
MLA：
su,hao,et al."Jiao-tai-wan inhibits inflammation of the gut-brain-axis and attenuates cognitive impairment in insomnic rats".JOURNAL OF ETHNOPHARMACOLOGY 250.(2020)
