单位：[1]Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Hubei, China[2]Department of Urology, the First Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang, China浙江大学医学院附属第一医院[3]College of Pharmacy, Hubei University of Science and Technology, Hubei, China[4]Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, China华中科技大学同济医学院附属协和医院[5]Wuhan Britain-China School, Hubei, China[6]Bio-X, Stanford University School of Medicine, Stanford, CA, USA[7]Department of Urology, Jianyang First Hospital, Fujian, China[8]Department of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Hubei,China外科学系华中科技大学同济医学院附属同济医院泌尿外科[9]Suzhou OptoMedic Technologies Inc, Jiangsu, China[10]School of Life Science and Technology, Wuhan Polytechnic University, Hubei, China
This study aimed to evaluate the combined efficacy of hyperthermia and chemotherapy using a bladder cancer organoid model and to explore hyperthermia-related molecular pathways.Tumor organoids were generated by embedding RT4 bladder cancer cells into Matrigel. The resulting organoids were treated with pirarubicin or gemcitabine at 37 °C or 42 °C. Proliferation was determined by Ki67 immunofluorescence staining, and apoptosis was assessed using a TdT-mediated dUTP nick end labeling (TUNEL) assay. RNA sequencing was used to identify the differentially expressed genes.Bladder cancer organoids were successfully established and exhibited robust proliferative abilities. Treatment with gemcitabine or pirarubicin under hyperthermic conditions caused pronounced structural damage to the organoids and increased cell death compared to that in the normothermically treated group. Furthermore, Ki67 labeling and TUNEL assays showed that the hyperthermia chemotherapy group showed a significantly reduced proliferation rate and high level of apoptosis. Finally, RNA sequencing revealed the IFN-γ signaling pathway to be associated with hyperthermia.Overall, hyperthermia combined with chemotherapy exerted better therapeutic effects than those of normothermic chemotherapy in grade 1-2 non-muscle-invasive bladder cancer, potentially through activation of the IFN-γ-JAK-STAT pathway.
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2023]版：
大类|3 区医学
小类|3 区肿瘤学3 区核医学
最新[2025]版：
大类|3 区医学
小类|3 区肿瘤学3 区核医学
JCR分区：
出版当年[2022]版：
Q2RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGINGQ3ONCOLOGY
最新[2023]版：
Q2ONCOLOGYQ2RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
第一作者单位：[1]Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Hubei, China
共同第一作者：
通讯作者：
通讯机构：[1]Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Hubei, China[*1]Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Hubei, China.
推荐引用方式(GB/T 7714)：
Xu Ying,Sun Guoliang,Yang Tiantian,et al.Validation of hyperthermia as an enhancer of chemotherapeutic efficacy: insights from a bladder cancer organoid model[J].INTERNATIONAL JOURNAL OF HYPERTHERMIA.2024,41(1):2316085.doi:10.1080/02656736.2024.2316085.
APA：
Xu Ying,Sun Guoliang,Yang Tiantian,Li Huaibiao,Hu Poyi...&Zhang Huiping.(2024).Validation of hyperthermia as an enhancer of chemotherapeutic efficacy: insights from a bladder cancer organoid model.INTERNATIONAL JOURNAL OF HYPERTHERMIA,41,(1)
MLA：
Xu Ying,et al."Validation of hyperthermia as an enhancer of chemotherapeutic efficacy: insights from a bladder cancer organoid model".INTERNATIONAL JOURNAL OF HYPERTHERMIA 41..1(2024):2316085
