单位：[1]Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China口腔科华中科技大学同济医学院附属同济医院[2]School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.[3]The Dental College of Georgia, Augusta University, Augusta, GA, USA.
To evaluate the bacteria debridement efficacy of two generations of sonic root canal irrigant activation systems: EndoActivator (Dentsply Sirona), the first generation, and SmartLite Pro EndoActivator, the second generation.Instrumented, autoclaved, single-rooted human premolars were inoculated with Enterococcus faecalis (ATCC-29212) for 21 days. The bacteria biofilm-containing teeth were randomly divided into 5 groups (N=8): Group 1: Syringe-side-vented needle (S-N) delivery of saline for 1 min; Group 2: S-N delivery of 2% NaOCl for 1 min; Group 3: S-N delivery of 2% NaOCl for 5 min; Group 4: EndoActivator activation of 2% NaOCl for 1 min; Group 5: SmartLite Pro EndoActivator activation of 2% NaOCl for 1 min. The teeth were evaluated for bacterial reduction using CFU counts, and the percentages of dead bacteria within the dentinal tubules using confocal laser scanning microscopy.Activation of NaOCl with EndoActivator or SmartLite Pro EndoActivator significantly reduced the overall intracanal bacterial load, compared with S-N irrigant delivery (P<0.05), with no significant difference between the two agitation devices (P>0.05). Nevertheless, S-N delivery of 2% NaOCl for 5 min produced better bacteria debridement than either sonic agitation system. Different degrees of bacteria kill were identified in the coronal-middle portions and apical portion of the canal space.Delivery time of NaOCl affects the efficacy of bacteria disinfection. Activation for 1 min with the EndoActivator or SmartLite Pro EndoActivator demonstrated comparable canal wall biofilm and intracanal bacteria reduction efficacy when 2% NaOCl was used as irrigant for disinfecting E. faecalis in single-rooted teeth.Although the sonic root canal irrigant activation devices investigated do not completely eliminate live bacteria biofilms from the canal space, they help reduce bacteria load during irrigant activation.Published by Elsevier Ltd.
基金：
This work was supported by National Natural Science Foundation of China (grant number: 81873714), National Natural Science Foundation of China (grant number: 62171193), and Key Research and Development Project of Hubei Province of China (grant number: 2022BCA033).
第一作者单位：[1]Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China[2]School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China[2]School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
推荐引用方式(GB/T 7714)：
Zeng Chang,Hu Pei,Egan Colin P,et al.Bacteria debridement efficacy of two sonic root canal irrigant activation systems[J].JOURNAL OF DENTISTRY.2024,140:doi:10.1016/j.jdent.2023.104770.
APA：
Zeng Chang,Hu Pei,Egan Colin P,Bergeron Brian E,Tay Franklin&Ma Jingzhi.(2024).Bacteria debridement efficacy of two sonic root canal irrigant activation systems.JOURNAL OF DENTISTRY,140,
MLA：
Zeng Chang,et al."Bacteria debridement efficacy of two sonic root canal irrigant activation systems".JOURNAL OF DENTISTRY 140.(2024)
医学