单位：[1]State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China[2]School of Food Science and Technology, Nanchang University, Nanchang 330047, China[3]Department of Allergology, Zhongnan Hospital of Wuhan University, Wuhan, China[4]Department of Allergy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China过敏反应科华中科技大学同济医学院附属同济医院[5]Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang, 330047, China[6]Sino-German Joint Research Institute (Jiangxi-OAI), Nanchang University, Nanchang 330047, China
Cow milk is an important source of food protein for children, however, it could lead to allergy, especially for infant. α-lactalbumin (α-LA) and β-lactoglobulin (β-LG) from whey protein make up a relatively high proportion of milk proteins and have received widespread attention as major allergens in milk. However, few studies have identified the epitopes of both proteins simultaneously. In this study, ImmunoCAP and indirect ELISA were first used for detection of sIgE to screen sera from allergic patients with high binding capacity for α-LA and β-LG. Subsequently, the mimotopes was biopanned by phage display technology and bioinformatics and 17 mimic peptide sequences were obtained. Aligned with the sequences of α-LA or β-LG, we identified one linear epitope on α-LA at AA11-26 and 5 linear epitopes on β-LG at AA 9-29, AA 45-57, AA 77-80, AA 98-101, and AA 121-135, respectively. Meanwhile, the 8 conformational epitopes and their distributions of α-LA and β-LG were located using the Pepitope Server espectively. Finally, Glutamine and lysine were determined as common AA residues for the conformational epitopes both on α-LA and β-LG. Moreover, we found the addition of mouse antihuman IgE during the biopanning process did not significantly affect the identification of the epitopes.The Authors. Published by Elsevier Inc. and Fass Inc. on behalf of the American Dairy Science Association®. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
基金：
This research was funded by the National Natural
Science Foundation of China (32072241) and Special
Project for Research and Development in Key areas of
Guangdong Province (2022B0202030002). The authors
have not stated any conflicts of interest.
第一作者单位：[1]State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China[2]School of Food Science and Technology, Nanchang University, Nanchang 330047, China
通讯作者：
通讯机构：[1]State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China[2]School of Food Science and Technology, Nanchang University, Nanchang 330047, China[5]Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang, 330047, China
推荐引用方式(GB/T 7714)：
Wang Xinyu,Hu Yongxin,Tan Hongkai,et al.Glutamine and lysine as common residues from epitopes on α-lactalbumin and β-lactoglobulin from cow milk identified by phage display technology[J].JOURNAL OF DAIRY SCIENCE.2023,106(11):7382-7395.doi:10.3168/jds.2022-23151.
APA：
Wang Xinyu,Hu Yongxin,Tan Hongkai,Dong Xiang,Zhang Shuchen...&Li Xin.(2023).Glutamine and lysine as common residues from epitopes on α-lactalbumin and β-lactoglobulin from cow milk identified by phage display technology.JOURNAL OF DAIRY SCIENCE,106,(11)
MLA：
Wang Xinyu,et al."Glutamine and lysine as common residues from epitopes on α-lactalbumin and β-lactoglobulin from cow milk identified by phage display technology".JOURNAL OF DAIRY SCIENCE 106..11(2023):7382-7395
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