MicroRNA (miR)-145 is enriched in the follicular granulosa cells (GCs) of 3-week-old mice. Downregulating miR-145 inhibits the proliferation and differentiation of GCs and induces evident changes in their cytoskeleton. In this study, we examined how miR-145 induces cytoskeletal changes in mouse GCs and its potential mechanism in regulating GC steroidogenesis. We found that actin related protein 2/3 complex subunit 5 (Arpc5) is a target of miR145. The miR-145 antagomir increased ARPC5 expression but not ss-ACTIN, ss-TUBULIN, and PAXILLIN expression. Arpc5 overexpression inhibited GC proliferation, differentiation, and progesterone synthesis. Furthermore, the expression of progesterone synthesis-associated enzymes was downregulated in the Arpc5 overexpression group, and the GC cytoskeleton exhibited evident changes. We conclude that Arpc5, a new target of miR-145, regulates primary GC proliferation and progesterone production by regulating the cytoskeleton remodeling.
基金：
Guizhou Provincial Science and Technology Projects [ZK [2022] YB-004]; National Natural Science Foundation of China [81501227, 81701438, 81902669]
第一作者单位：[1]Guiyang Matern & Child Hlth Care Hosp, Dept Obstet & Gynecol, Guizhou 550003, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Ma Lanfang,Wang Shuo,Yang Jun,et al.MiR-145 regulates steroidogenesis in mouse primary granulosa cells by targeting Arpc5 and subsequent cytoskeleton remodeling[J].JOURNAL OF REPRODUCTION AND DEVELOPMENT.2023,69(3):154-162.doi:10.1262/jrd.2022-137.
APA：
Ma, Lanfang,Wang, Shuo,Yang, Jun,Tang, Weicheng,Wu, Zhangying...&Wang, Shixuan.(2023).MiR-145 regulates steroidogenesis in mouse primary granulosa cells by targeting Arpc5 and subsequent cytoskeleton remodeling.JOURNAL OF REPRODUCTION AND DEVELOPMENT,69,(3)
MLA：
Ma, Lanfang,et al."MiR-145 regulates steroidogenesis in mouse primary granulosa cells by targeting Arpc5 and subsequent cytoskeleton remodeling".JOURNAL OF REPRODUCTION AND DEVELOPMENT 69..3(2023):154-162
