单位：[1]Department of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology.华中科技大学同济医学院附属同济医院外科学系泌尿外科[2]Department of Urology, The First Affiliated Hospital of Zhengzhou University.[3]Department of Ultrasound, The First Affiliated Hospital of Zhengzhou University.[4]Reproductive Medicine Center,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology.妇产科学系计划生育专科华中科技大学同济医学院附属同济医院
Erectile dysfunction and atherosclerosis are common cardiovascular complications in diseases. Clinical associations between erectile dysfunction and atherosclerosis have been noticed, but the specific mechanisms are not illustrated adequately.The aim of the study was to further mine associated pathological mechanisms and genetic alterations of atherosclerosis in diabetes mellitus-related erectile dysfunction (DMED).Significant atherosclerosis-related genes (ASRGs) were identified from transcriptome data of DMED and ASRG sets from DisGeNET and GeneCard databases. Functional enrichment and immune infiltration analyses were performed to clarify the biological roles and pathways as well as immune responses of significant ASRGs. A protein-protein interaction (PPI) network was constructed and gene clusters were performed. Then, data of diabetic plaques and high-glucose cavernosum endothelial cells was analyzed for validation. And hub ASRGs were identified. Finally expressed pattern of hub ASRGs were explored by single-cell profiling and immune analysis.In total, 202 significant ASRGs including 100 up-regulated and 102 down-regulated genes were identified. These genes were related to endothelial cell migration, inflammatory response, regulation of oxidative stress and immune response. In immune infiltration, immature dendritic cell and monocyte showed differential expression between the DMED and control groups, A PPI network containing 135 nodes was constructed. A hub-ASRG signature consisting of HBEGF, LOX, NQO1 and VLDLR was obtained by multi-omics validation. In addition, Functional enrichment analysis revealed that hub ASRGs were involved in oxidoreductase activity and extracellular matrix organization.We explored atherosclerosis-related genetic changes and signaling pathways in DMED. HBEGF, LOX, NQO1 and VLDLR were identified as hub ASRGs. These may serve as potential biomarkers for the clinical management of atherosclerosis and preventing further cardiovascular risks in DMED. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.
基金：
National Natural Science Foundation of China (grant
number 82201775 and 82201758) and the Medical Science and Technology Research-related joint
construction project of Henan Province (No. LHGJ20220343)
第一作者单位：[1]Department of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology.[2]Department of Urology, The First Affiliated Hospital of Zhengzhou University.
通讯作者：
通讯机构：[1]Department of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology.[2]Department of Urology, The First Affiliated Hospital of Zhengzhou University.
推荐引用方式(GB/T 7714)：
Yuan Penghui,Sun Taotao,Han Zhengyang,et al.Identifying potential cross-talk signatures for the occurrence of atherosclerosis in diabetic erectile dysfunction[J].ANDROLOGY.2023,11(6):1031-1043.doi:10.1111/andr.13366.
APA：
Yuan Penghui,Sun Taotao,Han Zhengyang&Chen Yinwei.(2023).Identifying potential cross-talk signatures for the occurrence of atherosclerosis in diabetic erectile dysfunction.ANDROLOGY,11,(6)
MLA：
Yuan Penghui,et al."Identifying potential cross-talk signatures for the occurrence of atherosclerosis in diabetic erectile dysfunction".ANDROLOGY 11..6(2023):1031-1043
