单位：[1]Department of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,No. 1095 Jie Fang Avenue,Wuhan,Hubei 430030,China华中科技大学同济医学院附属同济医院外科学系泌尿外科[2]Institute of Urology of Hubei Province, No. 1095 Jie Fang Avenue, Wuhan, Hubei 430030, China
We have previously demonstrated that miR-U80-5p has potent ability to upregulate p21 expression by targeting promoter and inhibit bladder cancer. This prompted us to conjecture that a candidate dsRNA (dsP21-397) with perfect complementarity to the miR-1180-5p target site of p21 promoter may also trigger p21 expression. Transfection of dsP21-397 into T24 and EJ cells significantly activated p21 expression at 72 h and the activation presented in a time-course and dose-dependent manner. Moreover, the p21-activated activities of dsP21-397 and miR-U80-5p are not significantly different. Overexpression of p21 downregulated Cyclin Dl, CDK4/6, and Cydin A2 expression, and thereby induced cell cyde arrest and inhibited proliferation. Moreover, dsP21-397 suppressed bladder cancer largely depended on manipulating p21. In conclusion, our study identifies a pair of miRNA-dsRNA mediating endogenous p21 overexpression.
基金：
National Natural Science
Foundation of China (Grant no. 81372759), China
语种：
外文
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2014]版：
大类|3 区生物
小类|3 区生物工程与应用微生物3 区医学：研究与实验
最新[2025]版：
大类|4 区医学
小类|4 区生物工程与应用微生物4 区医学：研究与实验
JCR分区：
出版当年[2013]版：
Q4BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ4MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者单位：[1]Department of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,No. 1095 Jie Fang Avenue,Wuhan,Hubei 430030,China[2]Institute of Urology of Hubei Province, No. 1095 Jie Fang Avenue, Wuhan, Hubei 430030, China
通讯作者：
通讯机构：[1]Department of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,No. 1095 Jie Fang Avenue,Wuhan,Hubei 430030,China[2]Institute of Urology of Hubei Province, No. 1095 Jie Fang Avenue, Wuhan, Hubei 430030, China
推荐引用方式(GB/T 7714)：
Chenghe Wang,Qiangqiang Ge,Zhong Chen,et al.A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21~(Wafl/CIP1) Expression[J].BioMed research international.2015,(15):doi:10.1155/2015/304753.
APA：
Chenghe Wang,Qiangqiang Ge,Zhong Chen,Jia Hu,Fan Li...&Zhangqun Ye.(2015).A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21~(Wafl/CIP1) Expression.BioMed research international,,(15)
MLA：
Chenghe Wang,et al."A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21~(Wafl/CIP1) Expression".BioMed research international ..15(2015)
