Metalloporphyrin and gold nanoparticles modified hollow zeolite imidazole Framework-8 with excellent peroxidase like activity for quick colorimetric determination of choline in infant formula milk powder
Metalloporphyrin and gold nanoparticles (AuNPs) were fixed on the surface of hollow zeolite imidazole framework-8 (HZIF-8) by cation exchange and cross-linking reaction. The obtained hybrid nanozyme, Au/HZIF-8@TCPP(Fe), was fully characterized by TEM, HRTEM, EDS element mapping and XPS. Then, its peroxidase-like activity was explored with Km of 1.74 mM and Vmax of 9.60 x 10(-8) M.S-1 towards H2O2, indicating excellent catalytic activity. Based on cascade reaction between choline oxidase and Au/HZIF-8@TCPP(Fe), a quick colorimetric method was established for choline detection in infant formula milk powder. After comprehensive verification, this method presented the merits of simple operation, satisfied detection limit (0.05 mM), wide linear range (0.05-2.0 mM), high accuracy (recovery of 92.2%-105.2%) and nice selectivity. This colorimetric method was applied to the determination of choline in milk powders of five brands. Our study could offer valuable reference for finding highly efficient nanozyme and constructing novel optical biosensors.
基金：
Department of Education of Hubei Province [D20201604]; Hubei Key Laboratory for Processing and Transformation of Agricultural Products (Wuhan Poly-technic University) [2019HBSQGDKFB03]
第一作者单位：[1]Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China[*1]Xuefu South Rd 68, Wuhan, Hubei, Peoples R China[*2]1095 Jiefang Ave, Wuhan, Hubei, Peoples R China
通讯作者：
通讯机构：[1]Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China[2]Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Lab Med, Wuhan 430030, Peoples R China[*1]Xuefu South Rd 68, Wuhan, Hubei, Peoples R China[*2]1095 Jiefang Ave, Wuhan, Hubei, Peoples R China
推荐引用方式(GB/T 7714)：
Zhang Haizhi,Wu Hongyuan,Qin Xinguang,et al.Metalloporphyrin and gold nanoparticles modified hollow zeolite imidazole Framework-8 with excellent peroxidase like activity for quick colorimetric determination of choline in infant formula milk powder[J].FOOD CHEMISTRY.2022,384:doi:10.1016/j.foodchem.2022.132552.
APA：
Zhang, Haizhi,Wu, Hongyuan,Qin, Xinguang,Shen, Ying,Wei, Xinlin&Liu, Gang.(2022).Metalloporphyrin and gold nanoparticles modified hollow zeolite imidazole Framework-8 with excellent peroxidase like activity for quick colorimetric determination of choline in infant formula milk powder.FOOD CHEMISTRY,384,
MLA：
Zhang, Haizhi,et al."Metalloporphyrin and gold nanoparticles modified hollow zeolite imidazole Framework-8 with excellent peroxidase like activity for quick colorimetric determination of choline in infant formula milk powder".FOOD CHEMISTRY 384.(2022)
农林科学