Loop-mediated isothermal amplification (LAMP) is widely used in detection of pathogenic microorganisms including SARS-CoV-2. However, the performance of LAMP assay needs further exploration in the emerging SARS-CoV-2 variants test. Here, we design serials of primers and select an optimal set for LAMP-based on SARS-CoV-2 N gene for a robust and visual assay in SARS-CoV-2 diagnosis. The limit of detectable template reaches 10 copies of N gene per 25 mu L reaction at isothermal 58? within 40 min. Importantly, the primers for LAMP assay locate at 12 to 213 nt of N gene, a highly conservative region, which serves as a compatible test in emerging SARS-CoV-2 variants. Comparison to a commercial qPCR assay, this LAMP assay exerts the high viability in diagnosis of 41 clinical samples. Our study optimizes an advantageous LAMP assay for colorimetric detection of SARS-CoV-2 and emerging variants, which is hopeful to be a promising test in COVID-19 surveillance. (C) 2022 Elsevier Ltd. All rights reserved.
基金：
National Natural Science Foundation of China [32070148]; Guangzhou Basic Research Program-Basic and Applied Basic Research Project [202102020260]
第一作者单位：[1]Jinan Univ, Inst Med Microbiol, Guangdong Prov Key Lab Virol, Guangzhou 510632, Peoples R China[2]Foshan Inst Med Microbiol, Foshan 528315, Peoples R China
通讯作者：
通讯机构：[1]Jinan Univ, Inst Med Microbiol, Guangdong Prov Key Lab Virol, Guangzhou 510632, Peoples R China[2]Foshan Inst Med Microbiol, Foshan 528315, Peoples R China[4]Guangdong Longfan Biol Sci & Technol Co, Foshan 528315, Peoples R China
推荐引用方式(GB/T 7714)：
Luo Zhen,Ye Chunhong,Xiao Heng,et al.Optimization of loop-mediated isothermal amplification (LAMP) assay for robust visualization in SARS-CoV-2 and emerging variants diagnosis[J].CHEMICAL ENGINEERING SCIENCE.2022,251:doi:10.1016/j.ces.2022.117430.
APA：
Luo, Zhen,Ye, Chunhong,Xiao, Heng,Yin, Jialing,Liang, Yicong...&Wu, Jianguo.(2022).Optimization of loop-mediated isothermal amplification (LAMP) assay for robust visualization in SARS-CoV-2 and emerging variants diagnosis.CHEMICAL ENGINEERING SCIENCE,251,
MLA：
Luo, Zhen,et al."Optimization of loop-mediated isothermal amplification (LAMP) assay for robust visualization in SARS-CoV-2 and emerging variants diagnosis".CHEMICAL ENGINEERING SCIENCE 251.(2022)
工程技术