单位：[1]Institute of Public Health, Henan University, Kaifeng 475004, China[2]Cancer Research Institute, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China华中科技大学同济医学院附属同济医院胃肠肿瘤研究所
Abstract Objective: The aim of the study was to observe the expression of Bcl-2 and its phosphorylation in Molt-4 cells
induced by tumor necrosis factor-α (TNF-α), and to investigate the possible mechanism of cell cycle specificity of apoptosis.
Methods: Exponentially growing Molt-4 cells were treated with TNF-α. Apoptosis was detected by DNA fragmentation assay.
API method was applied to illustrate the cell cycle specificity of apoptotic cells. Cells of sub-phases were sorted by FACSvantage
flow cytometer and then submitted to immunoblot. Results: Molt-4 cells which were treated with TNF-α went to apoptosis
and showed a DNA ladder pattern. Most apoptosis happened in G1-phase of cell cycle. Bcl-2 expression increased for the
Molt-4 cells treated with TNF-α. The phosphorylation state of Bcl-2 was only presented in G1-phase cells, in accordance with
the specified time and cell cycle phase of apoptosis. Conclusion: The phosphorylation of Bcl-2 in the Molt-4 cells treated with
TNF-α happened with the same cell cycle specificity as cell apoptosis. The cell cycle specificity of Bcl-2 phosphorylation was
one of the mechanisms of receptor-mediated apoptosis. The cell cycle machine can trigger the apoptosis program.
基金：
Clinical Key Subject Foundation of Health
Ministry of China (No. 20012537) and National Science and Technology
Department “973” Tumor Project (No. 2004CB518705).
语种：
外文
第一作者：
第一作者单位：[1]Institute of Public Health, Henan University, Kaifeng 475004, China
通讯作者：
推荐引用方式(GB/T 7714)：
Changyong Yang,Huijie Zhao,Jianping Gong.TNF-a induces apoptosis of Molt-4 cells and cell cycle specificity of Bcl-2 phosphorylation[J].CHINESE-GERMAN JOURNAL OF CLINICAL ONCOLOGY.2010,
APA：
Changyong Yang,Huijie Zhao&Jianping Gong.(2010).TNF-a induces apoptosis of Molt-4 cells and cell cycle specificity of Bcl-2 phosphorylation.CHINESE-GERMAN JOURNAL OF CLINICAL ONCOLOGY,,
MLA：
Changyong Yang,et al."TNF-a induces apoptosis of Molt-4 cells and cell cycle specificity of Bcl-2 phosphorylation".CHINESE-GERMAN JOURNAL OF CLINICAL ONCOLOGY .(2010)
