单位：[1]Department of Rehabilitation,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China华中科技大学同济医学院附属同济医院康复医学科[2]Department of Neurology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China华中科技大学同济医学院附属同济医院神经科[3]School of Mechanical Engineering, Hubei University of Technology, Wuhan 430068, China[4]Waisman Center, Department of Neuroscience and Department of Neurology, University of Wisconsin, Madison, WI, USA[5]Program in Neuroscience & Behavioral Disorders, Duke-NUS Medical School, Singapore, Singapore
Spinal cord injury (SCI) often results in spasticity. There is currently no effective therapy for spasticity. Here, we describe a method to efficiently differentiate human pluripotent stem cells from spinal GABA neurons. After transplantation into the injured rat spinal cord, the DREADD (designer receptors exclusively activated by designer drug)-expressing spinal progenitors differentiate into GABA neurons, mitigating spasticity-like response of the rat hindlimbs and locomotion deficits in 3 months. Administering clozapine-N-oxide, which activates the grafted GABA neurons, further alleviates spasticity-like response, suggesting an integration of grafted GABA neurons into the local neural circuit. These results highlight the therapeutic potential of the spinal GABA neurons for SCI.
基金：
National Key R&D Program of China [2016YFA0102500]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81471302]; Bleser Family Foundation; Busta Family Foundation for Spinal Cord Injury Research
第一作者单位：[1]Department of Rehabilitation,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China
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推荐引用方式(GB/T 7714)：
Gong ChenZi,Zheng Xiaolong,Guo FangLiang,et al.Human spinal GABA neurons alleviate spasticity and improve locomotion in rats with spinal cord injury[J].CELL REPORTS.2021,34(12):doi:10.1016/j.celrep.2021.108889.
APA：
Gong,ChenZi,Zheng,Xiaolong,Guo,FangLiang,Wang,YaNan,Zhang,Song...&Chen,Hong.(2021).Human spinal GABA neurons alleviate spasticity and improve locomotion in rats with spinal cord injury.CELL REPORTS,34,(12)
MLA：
Gong,ChenZi,et al."Human spinal GABA neurons alleviate spasticity and improve locomotion in rats with spinal cord injury".CELL REPORTS 34..12(2021)
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