K+ cycling in the cochlea is critical to maintain hearing. Many sodium-potassium pumps are proved to participate in K+ cycling, such as Na/K-ATPase. The alpha 2-Na/K-ATPase is an important isoform of Na/K-ATPase. The expression of alpha 2-Na/K-ATPase in the cochlea is not clear. In this study, we used C57BL/6 mice as a model of presbycusis and implemented immunohistochemistry staining and quantitative real time-PCR, and the alpha 2-Na/K-ATPase expression pattern was confirmed in the inner ear. It was found alpha 2-Na/K-ATPase was expressed widely in cochlea and its mRNA and protein expression was gradually reduced with aging (4-, 14-, 26- and 48-weeks old mice). We suspected that, the down-regulation of alpha 2-Na/K-ATPase expression might be associated with the remodeling of K+ cycling, degeneration of morphological structure and decrease of hearing function in aging C57 mice. In conclusion, we speculated that the reduction of alpha 2-Na/K-ATPase might play an important role in the pathogenesis of age-related hearing loss.
基金：
National Natural Science Foundation of China [81771004, 81271078, 81500791, 81500794, 81300827]
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Otolaryngol Head & Neck Surg, Tongji Hosp, Wuhan 430030, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Liu Yun,Chu Han-qi,Sun Yan-bo,et al.Expression of α2-Na/K-ATPase in C57BL/6J Mice Inner Ear and Its Relationship with Age-related Hearing Loss[J].CURRENT MEDICAL SCIENCE.2021,41(1):153-157.doi:10.1007/s11596-021-2330-5.
APA：
Liu, Yun,Chu, Han-qi,Sun, Yan-bo,Bing, Dan,Zhou, Liang-qiang...&Du, Zhi-hui.(2021).Expression of α2-Na/K-ATPase in C57BL/6J Mice Inner Ear and Its Relationship with Age-related Hearing Loss.CURRENT MEDICAL SCIENCE,41,(1)
MLA：
Liu, Yun,et al."Expression of α2-Na/K-ATPase in C57BL/6J Mice Inner Ear and Its Relationship with Age-related Hearing Loss".CURRENT MEDICAL SCIENCE 41..1(2021):153-157
