Background: The aim of the present study was to investigate the effect of over-expressing circular RNA (circ_0003645) on cell functions and its molecular mechanism in breast cancer. Methods: The expression profile of circ_0003645, breast cancer cell lines, and the transcription levels of circular RNA, miRNA and HMGB1 gene were detected by qRT-PCR. Flow cytometry analysis was manipulated to evaluate cancer cell proliferation and cell apoptosis. The correlation between miR-139p-3p and circular_0003645 or HMGB1 was predicted by GEO, and TCGA was confirmed using the dual-luciferase reporter assay. Results: Circ_0003645 expression was conspicuously increased in both the breast cancer tissues and cell lines. Circ_0003645 knockdown inhibited cell proliferation and induced the apoptosis of breast cancer cells in vitro and in vivo. By sponging miR-139-3p, circ_0003645 promoted the breast cancer cells progression and positively regulated HMGB1 gene. Conclusion: Circ_0003645 functions as a ceRNA for miR-139-3p, which could upregulate HMGB1 and further promote cell proliferation in breast cancer.
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Hosp, Dept Breast & Thyroid Surg, Tongji Med Coll, Wuhan 430030, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang Jinjun,Ke Shun,Zheng Weihong,et al.Hsa_circ_0003645 Promotes Breast Cancer Progression by Regulating miR-139-3p/HMGB1 Axis[J].ONCOTARGETS AND THERAPY.2020,13:10361-10372.doi:10.2147/OTT.S265796.
APA：
Zhang, Jinjun,Ke, Shun,Zheng, Weihong,Zhu, Zhi&Wu, Yanle.(2020).Hsa_circ_0003645 Promotes Breast Cancer Progression by Regulating miR-139-3p/HMGB1 Axis.ONCOTARGETS AND THERAPY,13,
MLA：
Zhang, Jinjun,et al."Hsa_circ_0003645 Promotes Breast Cancer Progression by Regulating miR-139-3p/HMGB1 Axis".ONCOTARGETS AND THERAPY 13.(2020):10361-10372
