单位：[a]Dept. of Respiratory Medicine, Renmin Hospital, Wuhan University, Wuhan 430060, China[b]Dept. of Respiratory Medicine, Tongji Hospital of Tongji Medical College, Huazhong Science and Technology University, Wuhan 430030, China华中科技大学同济医学院附属同济医院内科学系呼吸与危重症医学科
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摘要：
Objective: To study the effect of the phosphoinositide 3 kinase (PI-3K) expression on the proliferation of airway smooth muscle cells (ASMCs) in rats with chronic obstructive pulmonary disease (COPD) induced by smoking. Methods: The rat models of COPD induced by smoking were made to investigate the resistance of airway and the total compliance of respiratory system. The expression of PCNA in ASMCs was determined by immunohistochemistry, and the mRNA expression of PI-3K in ASMCs was determined by reverse transcription polymerase chain reaction (RT-PCR). Results: The resistance of airway was increased in the COPD groups as compared with control group, and the expressions of PCNA and PI-3K in ASMCs of COPD groups were significantly increased (all P<0. 05, n=15). The expression level of PI-3K had strongly positive correlation with the expression level of PCNA (r=0. 856, P<0. 05, n=15). Conclusion: The PI-3K signal pathway may be involved in ASMCs proliferation in COPD rats induced by smoking.
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中文
第一作者：
第一作者单位：[a]Dept. of Respiratory Medicine, Renmin Hospital, Wuhan University, Wuhan 430060, China
推荐引用方式(GB/T 7714)：
Chen H,Xu Y,Xie J,等.Role of phosphoinositide 3 Kinases in airway smooth muscle cells proliferation in COPD model rats induced by smoking[J].武汉大学学报(医学版).2010,31(4):
