单位：[a]Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China华中科技大学同济医学院附属同济医院消化内科[b]Hainan Provincial People's Hospital, Haikou 570311, Hainan Province, China
AIM: To investigate the impact of small interfering RNA (siRNA)-mediated inhibition of nuclear factor-κB P65 (NF-κB p65) on Bcl-2 expression and apoptosis in human hepatocellular carcinoma (HCC) cell lines. METHODS: HCC cell lines HepG2, SMMC7721 and human fetal liver cell line LO2 were used in the study. The expression of NF-κB p65 and Bcl-2 in the above three cell lines was detected by Western blot. SiRNA technology was then used to inhibit NF-κB p65 to observe the effect of NF-κB p65 knockdown on Bcl-2 expression and cell apoptosis. RESULTS: The expression levels of NF-κB p65 and Bcl-2 in HepG2 and SMMC7721 cells were significantly higher than those in LO2 cells (2.14 ± 0.19, 2.09 ± 0.27 vs 0.54 ± 0.11; 1.42 ± 0.15, 1.47 ± 0.14 vs 0.60 ± 0.08, all P < 0.05). No significant difference was detected in the expression levels of NF-κB p65 and Bcl-2 between HepG2 and SMMC7721 cells. SiRNA transfection significantly down-regulated NF-κB p65 expression in HepG2 and SMMC7721 cells compared to nontransfected cells (2.08 ± 0.19 vs 0.99 ± 0.12; 2.03 ± 0.17 vs 0.94 ± 0.14, both P < 0.05). SiRNA-mediated NF-κB p65 knockdown significantly down regulated Bcl-2 expression (1.37 ± 0.05 vs 0.72 ± 0.02; 1.44 ± 0.03 vs 0.69 ± 0.03, both P < 0.05) and increased apoptosis (5.12% ± 0.61% vs 37.87% ± 4.10%; 5.80% ± 0.71% vs 40.19% ± 3.78%, both P <0.05) in HepG2 and SMMC7721 cells compared to non-transfected cells. CONCLUSION: SiRNA-mediated NF-κB p65 knockdown significantly down-regulates Bcl-2 expression and promotes apoptosis in HepG2 and SMMC7721 cells.
语种：
中文
第一作者：
第一作者单位：[a]Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China[b]Hainan Provincial People's Hospital, Haikou 570311, Hainan Province, China
通讯作者：
通讯机构：[a]Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China[b]Hainan Provincial People's Hospital, Haikou 570311, Hainan Province, China
推荐引用方式(GB/T 7714)：
Zhao X.-K,Ning Q.-M,Sun X.-N,等.SiRNA-mediated inhibition of NF-κb p65 down-regulations Bcl-2 expression and promotes apoptosis in hepatocellular carcinoma cell lines[J].世界华人消化杂志.2011,19(22):
