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Identification of key genes associated with the progression of intrahepatic cholangiocarcinoma using weighted gene co-expression network analysis

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收录情况： ◇ SCIE

作者：
ZI YE[1,2] * ; ZHIRUI ZENG[3,4] * ; DA WANG[5] * ; SHAN LEI[3,4] ; YIYI SHEN[6] ; ZUBING CHEN[1,2,*1] ;
单位： [1]Departments of General Surgery,Renmin Hospital of Wuhan University, Wuhan, Hubei 430060 [2]Departments of Hepatobiliary Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060 [3]Guizhou Provincial Key Laboratory of Pathogenesis and Drug Research on Common Chronic Diseases [4]Department of Physiology, School of Basic Medicine, Guizhou Medical University, Guiyang, Guizhou 550009 [5]Department of Biliary‑Pancreatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430060 [6]Department of Liver‑Biliary Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550009, P.R. China
出处：
DOI： 10.3892/ol.2020.11600
ISSN：

关键词： Hub genes Intrahepatic cholangiocarcinoma Weighted gene co-expression network analysis

摘要：
The present study aimed to identify the key genes that are associated with the progression of intrahepatic cholangiocarcinoma through weighted gene co-expression network analysis (WGCNA). A total of three gene datasets were downloaded from the Gene Expression Omnibus database, including GSE107943, GSE119336 and GSE26566. Differentially expressed genes (DEGs) between intrahepatic cholangiocarcinoma tissues and adjacent liver tissues were identified using GSE107943, while tissue specific genes between bile duct and liver tissues were identified using GSE26566. Following the removal of tissue-specific genes, real DEGs were used to construct the WGCNA to investigate the association between gene modules and clinical traits. Following functional analysis, pathway enrichment analysis and the construction of a protein-protein interaction (PPI) network were performed, hub genes were selected and their diagnostic value was verified in GSE119336 using a receiver operating characteristic curve. Finally, the protein levels of the hub genes were also verified in intrahepatic cholangiocarcinoma tissues. A total of 1,643 real DEGs were identified and used to construct the WGCNA. Additionally, a total of seven co-expressed gene modules were identified following WGCNA, while genes in brown and yellow modules were identified to be associated with multiple clinical traits (the number of clinical traits >3) and used as key modules. A total of 63 core key module genes were subsequently identified, and it was indicated that these genes were most enriched in the nucleus (Gene Ontology term) and the cell cycle pathway (Kyoto Encyclopedia of Genes and Genomes term). Finally, a total of eight genes, including cyclin B1, cell division cycle 20, cell division cycle associated 8, cyclin dependent kinase 1, centrosomal protein 55, kinesin family member 2C, DNA topoisomerase IIα and TPX2 microtubule nucleation factor, exhibited the highest score in PPI analysis and had a high diagnostic value for intrahepatic cholangiocarcinoma. In addition, the protein levels of these genes were also revealed to be increased in most intrahepatic cholangiocarcinoma tissues. These eight genes may be used as novel biomarkers for the diagnosis of intrahepatic cholangiocarcinoma. © 2020 Spandidos Publications. All rights reserved.

基金：
The present study was supported by the Program For Tackling Key Problems in Science and Technology (grant. no. 2007AA301B35‑2), the Natural Science Foundation of Hubei Province of China (grant. no. 2010CDB06807) and the Important Project of Wuhan Administration of Science and Technology (grant. no. 2.008E+11).
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外文
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出版当年[2019]版：
大类 | 4 区 医学
小类 | 4 区 肿瘤学
最新[2025]版：
大类 | 4 区 医学
小类 | 4 区 肿瘤学
JCR分区：
出版当年[2018]版：
Q4 ONCOLOGY
最新[2023]版：
Q3 ONCOLOGY

影响因子： 最新[2023版] 最新五年平均 出版当年[2018版] 出版当年五年平均 出版前一年[2017版] 出版后一年[2019版]

第一作者：
第一作者单位： [1]Departments of General Surgery,Renmin Hospital of Wuhan University, Wuhan, Hubei 430060 [2]Departments of Hepatobiliary Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060
共同第一作者：
通讯作者：
通讯机构： [1]Departments of General Surgery,Renmin Hospital of Wuhan University, Wuhan, Hubei 430060 [2]Departments of Hepatobiliary Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060 [*1]Department of Hepatobiliary Surgery, Renmin Hospital of Wuhan University, 99 Zhangzhi Dong Road, Wuhan, Hubei 430060, P.R. China
推荐引用方式(GB/T 7714)：
ZI YE,ZHIRUI ZENG,DA WANG,et al.Identification of key genes associated with the progression of intrahepatic cholangiocarcinoma using weighted gene co-expression network analysis[J].ONCOLOGY LETTERS.2020,20(1):483-494.doi:10.3892/ol.2020.11600.
APA：
ZI YE,ZHIRUI ZENG,DA WANG,SHAN LEI,YIYI SHEN&ZUBING CHEN.(2020).Identification of key genes associated with the progression of intrahepatic cholangiocarcinoma using weighted gene co-expression network analysis.ONCOLOGY LETTERS,20,(1)
MLA：
ZI YE,et al."Identification of key genes associated with the progression of intrahepatic cholangiocarcinoma using weighted gene co-expression network analysis".ONCOLOGY LETTERS 20..1(2020):483-494

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