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Plasmid-based short hairpin RNA against cyclin D1 attenuated pulmonary vascular remodeling in smoking rats

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收录情况： ◇ SCIE

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单位： [1]Huazhong Univ Sci & Technol,Key Lab Pulm Dis,Minist Hlth China,Dept Resp Med,Tongji Hosp,Tongji Med Coll,Wuhan 430030,Peoples R China

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关键词： Cigarette smoke Cyclin D1 Pulmonary vascular remodeling

摘要：

Accumulating evidence indicated that smoking might directly induce pulmonary vascular remodeling at the initial stage of chronic obstructive pulmonary disease (COPD). However, the molecular mechanism underlying this process remains poorly understood. To investigate the role of cyclin D1 in pulmonary vascular remodeling, we constructed a plasmid-based short hairpin RNA (shRNA) to knock down the expression of cyclin D1 in smoking rats. Specific shRNA against cyclin D1 significantly prevented the cyclin D1 expression and the cell proliferation in rat pulmonary artery smooth muscle cells (rPASMCs). Furthermore, the plasmid-based shRNA successfully decreased the cyclin D1 protein in intra-pulmonary arteries of smoking rats and subsequently decreased the wall thickness of pulmonary vessels and the percentage of muscularized vessels. We conclude that the plasmid-based shRNA against cyclin D1 gene attenuated pulmonary vascular remodeling in smoking rats. Cyclin D1 might play a critical role in cigarette smoke-induced pulmonary vascular remodeling via regulating rPASMCs proliferation. (C) 2010 Published by Elsevier Inc.

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出版当年[2009]版：

大类 | 3 区医学

小类 | 3 区外周血管病

最新[2025]版：

大类 | 3 区医学

小类 | 3 区外周血管病

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出版当年[2008]版：

Q2 PERIPHERAL VASCULAR DISEASE

最新[2023]版：

Q2 PERIPHERAL VASCULAR DISEASE

影响因子： 2.9 最新[2023版] 3 最新五年平均 3 出版当年[2008版] 2.756 出版当年五年平均 2.365 出版前一年[2007版] 3.075 出版后一年[2009版]

第一作者：

第一作者单位： [1]Huazhong Univ Sci & Technol,Key Lab Pulm Dis,Minist Hlth China,Dept Resp Med,Tongji Hosp,Tongji Med Coll,Wuhan 430030,Peoples R China

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