Gangliosides GM1 is a good marker of membrane microdomains (lipid rafts) with important function in cellular activation processes. In this study we found that GM1 expression on CD4+ T cells and memory T cells (CD45RO/CD4) were dramatic increased after stimulation with phytohaemagglutinin in vitro. Next, we examined the GM1 expression on peripheral blood CD4+ T cells and CD8+ T cells from 44 patients with SLE and 28 healthy controls by flow cytometry. GM1 expression was further analyzed with serum soluble CD30 (sCD30), IL-10, TNF-alpha and clinical parameters. The mean fluorescence intensity of GM1 on CD4+ T cells from patients with SLE was significantly higher than those from healthy controls, but not on CD8+ T cells. Increased expression of GM1 was more marked on CD4+/CD45RO+ memory T cells from active SLE patients. Patients with SLE showed significantly elevated serum sCD30 and IL-10, but not TNF-alpha levels. In addition, we found that enhanced GM1 expression on CD4+ T cells from patients with SLE positively correlated with high serum levels of sCD30 and IgG as well as disease activity (SLEDAI scores). Our data suggested the potential role of aberrant lipid raft/GM1 on CD4+ T cells and sCD30 in the pathogenesis of SLE.
基金：
National Natutal Science Foundation of China [30840006, 30972706]; Tongji Hospital Research Foundation; Uehara Memorial FoundationUehara Memorial Foundation; Vehicle Racing Commemorative Foundation; Kanazawa Medical University Research Foundation [C2009-4]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2009]版：
大类|4 区生物
小类|4 区生物工程与应用微生物4 区医学：研究与实验
最新[2025]版：
无
JCR分区：
出版当年[2008]版：
Q2BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ2MEDICINE, RESEARCH & EXPERIMENTAL
第一作者单位：[1]Huazhong Univ Sci & Technol, Dept Rheumatol & Immunol, Tongji Hosp, Tongji Med Coll, Wuhan 430030, Hubei, Peoples R China[*1]Huazhong Univ Sci & Technol, Dept Rheumatol & Immunol, Tongji Hosp, Tongji Med Coll, 1095th Jiefang Ave, Wuhan 430030, Hubei, Peoples R China
通讯作者：
通讯机构：[1]Huazhong Univ Sci & Technol, Dept Rheumatol & Immunol, Tongji Hosp, Tongji Med Coll, Wuhan 430030, Hubei, Peoples R China[*1]Huazhong Univ Sci & Technol, Dept Rheumatol & Immunol, Tongji Hosp, Tongji Med Coll, 1095th Jiefang Ave, Wuhan 430030, Hubei, Peoples R China
推荐引用方式(GB/T 7714)：
Dong Lingli,Hu Shaoxian,Chen Fang,et al.Increased Expression of Ganglioside GM1 in Peripheral CD4+T Cells Correlates Soluble Form of CD30 in Systemic Lupus Erythematosus Patients[J].JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY.2010,doi:10.1155/2010/569053.
APA：
Dong, Lingli,Hu, Shaoxian,Chen, Fang,Lei, Xiaomei,Tu, Wei...&Umehara, Hisanori.(2010).Increased Expression of Ganglioside GM1 in Peripheral CD4+T Cells Correlates Soluble Form of CD30 in Systemic Lupus Erythematosus Patients.JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY,,
MLA：
Dong, Lingli,et al."Increased Expression of Ganglioside GM1 in Peripheral CD4+T Cells Correlates Soluble Form of CD30 in Systemic Lupus Erythematosus Patients".JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY .(2010)
